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利用 GGE 双标图和 AMMI 分析评价甜菜(Beta vulgaris L.)基因型的产量和稳定性。

Evaluation of yield and stability of sugar beet (beta vulgaris L.) genotypes using GGE biplot and AMMI analysis.

机构信息

Sugar Beet Seed Institute (SBSI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Sugar Beet Research Department, West Azarbaijan Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Urmia, Iran.

出版信息

Sci Rep. 2024 Nov 9;14(1):27384. doi: 10.1038/s41598-024-78659-x.

Abstract

Rhizomania is the most destructive sugar beet disease in the world, and in recent years, it has widespread in most of the sugar beet growing areas in Iran. Since the control of this soil-borne disease is a difficult task, the use of resistant genotypes is known as the best measure against the disease. The ultimate goal of sugar beet breeders is to produce genotypes that can be used in both infected and non-infected fields without any reduction in terms of yield and quality. Twenty-one sugar beet genotypes along with four controls were evaluated in randomized complete block design with four replications in fields with natural infection to rhizomania in five research stations. Important sugar beet traits including root yield, sugar yield, sugar content, and white sugar yield were evaluated for two years (2021 and 2022). For all traits, location was the main source of variation that spanned 33 to 55% of the total sum of the square followed by the location×year×genotype accounted for 3-40% of the variation. Based on the results of analysis of variance, multivariate stability parameters were computed to evaluate the genotypes' stability. The first two principal components (IPCA1 and IPCA2) generated by GGE biplot contributed for 31.3 and 17.5% difference in genotype×environment interaction for root yield, respectively. According to the GGE biplot, genotypes RM-11 and RM-12 were identified as the winning genotypes across environments for both root yield and white sugar yield traits whereas AMMI model identified RM-14 and RM-9 (for root yield) and RM-1 (for white sugar yield) as best genotypes. Based on the ideal genotype ranking, RM-11 and RM-10 were the best performer with a high mean yield as well as stability in the studied environments. The biplot rendered using the weighted average of absolute scores (WAASB) and root yield and white sugar yield identified RM-11 and RM-9 as superior genotypes in terms of yield and stability. The selected genotypes can be used in breeding programs to transfer the disease resistance and cultivar development.

摘要

黄矮病是世界上最具破坏性的甜菜病害,近年来已广泛分布在伊朗大多数甜菜种植区。由于这种土传病害的防治是一项艰巨的任务,因此利用抗性基因型被认为是防治该病的最佳措施。甜菜育种者的最终目标是生产出既能在感染区又能在非感染区使用的基因型,而不会对产量和质量造成任何影响。在五个研究站的自然感染黄矮病田间,采用随机完全区组设计,设 4 次重复,对 21 个甜菜基因型和 4 个对照进行了评价。两年(2021 年和 2022 年)对重要的甜菜性状,包括根产量、糖产量、糖含量和白砂糖产量进行了评估。所有性状的主要变异来源均为地点,占总平方和的 33-55%,其次是地点×年份×基因型,占 3-40%。根据方差分析的结果,计算了多元稳定性参数来评估基因型的稳定性。GGE 双标图生成的前两个主成分(IPCA1 和 IPCA2)分别解释了根产量基因型×环境互作差异的 31.3%和 17.5%。根据 GGE 双标图,RM-11 和 RM-12 两种基因型被鉴定为根产量和白砂糖产量性状在所有环境下的优势基因型,而 AMMI 模型则鉴定出 RM-14 和 RM-9(根产量)和 RM-1(白砂糖产量)为最佳基因型。根据理想基因型排名,RM-11 和 RM-10 是表现最好的基因型,在研究的环境中具有较高的平均产量和稳定性。利用加权平均绝对得分(WAASB)和根产量和白砂糖产量生成的双标图,鉴定出 RM-11 和 RM-9 为产量和稳定性较高的优势基因型。选择的基因型可用于育成计划,以转移抗病性和品种开发。

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