pBFK，一种用于通过同源重组进行基因缺失的新型热敏穿梭载体。
pBFK, a new thermosensitive shuttle vector for gene deletion by homologous recombination.
作者信息
Boukthir S, Gaudu P, Faili A, Kayal S
机构信息
CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France.
Inserm, CIC 1414, Rennes, France.
出版信息
Heliyon. 2023 May 29;9(6):e16720. doi: 10.1016/j.heliyon.2023.e16720. eCollection 2023 Jun.
Abstract
or Group A (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS genotype.
摘要
A组链球菌（GAS）是一种主要的人类病原体，对其进行基因操作仍是一项持续的挑战。我们构建了一种新的表达壮观霉素抗性的温度敏感质粒pBFK，该质粒适用于同源重组程序，以在A组链球菌中进行完整的基因缺失。本文中，采用pBFK的诱变策略在一种高毒力的A组链球菌基因型中进行。
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