Zhou Shun, Yang Qiuhong, Song Yi, Cheng Bo, Ai Xiaohui
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Hu Bei Province Engineering and Technology Research Center of Aquatic Product Quality and Safety, Wuhan 430223, China.
Antioxidants (Basel). 2023 Jun 16;12(6):1288. doi: 10.3390/antiox12061288.
This study aimed to investigate the potential adverse effects of the practical application of copper sulfate on yellow catfish () and to provide insights into the gill toxicity induced by copper sulphate. Yellow catfish were exposed to a conventional anthelmintic concentration of copper sulphate (0.7 mg/L) for seven days. Oxidative stress biomarkers, transcriptome, and external microbiota of gills were examined using enzymatic assays, RNA-sequencing, and 16S rDNA analysis, respectively. Copper sulphate exposure led to oxidative stress and immunosuppression in the gills, with increased levels of oxidative stress biomarkers and altered expression of immune-related differentially expressed genes (DEGs), such as IL-1β, IL4Rα, and CCL24. Key pathways involved in the response included cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway. The 16S rDNA analysis revealed copper sulphate altered the diversity and composition of gill microbiota, as evidenced by a significant decrease in the abundance of Bacteroidotas and Bdellovibrionota and a significant increase in the abundance of Proteobacteria. Notably, a substantial 8.5-fold increase in the abundance of was also observed at the genus level. Our findings demonstrated that copper sulphate induced oxidative stress, immunosuppression, and gill microflora dysbiosis in yellow catfish. These findings highlight the need for sustainable management practices and alternative therapeutic strategies in the aquaculture industry to mitigate the adverse effects of copper sulphate on fish and other aquatic organisms.
本研究旨在探讨硫酸铜实际应用对黄颡鱼的潜在不良影响，并深入了解硫酸铜诱导的鳃毒性。将黄颡鱼暴露于常规驱虫浓度的硫酸铜（0.7毫克/升）中7天。分别使用酶促测定、RNA测序和16S rDNA分析检测鳃的氧化应激生物标志物、转录组和外部微生物群。硫酸铜暴露导致鳃出现氧化应激和免疫抑制，氧化应激生物标志物水平升高，免疫相关差异表达基因（DEGs）如IL-1β、IL4Rα和CCL24的表达发生改变。参与该反应的关键途径包括细胞因子-细胞因子受体相互作用、NOD样受体信号通路和Toll样受体信号通路。16S rDNA分析表明，硫酸铜改变了鳃微生物群的多样性和组成，拟杆菌门和蛭弧菌门的丰度显著降低，变形菌门的丰度显著增加证明了这一点。值得注意的是，在属水平上也观察到丰度大幅增加了8.5倍。我们的研究结果表明，硫酸铜会诱导黄颡鱼产生氧化应激、免疫抑制和鳃微生物群失调。这些发现凸显了水产养殖业需要可持续管理实践和替代治疗策略，以减轻硫酸铜对鱼类和其他水生生物的不利影响。
