Akache Bassel, Read Andrew J, Dudani Renu, Harrison Blair A, Williams Dean, Deschatelets Lise, Jia Yimei, Chandan Vandana, Stark Felicity C, Agbayani Gerard, Makinen Shawn R, Hemraz Usha D, Lam Edmond, Régnier Sophie, Zou Wei, Kirkland Peter D, McCluskie Michael J
National Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, Canada.
Virology Laboratory, Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2567, Australia.
Vaccines (Basel). 2023 May 31;11(6):1043. doi: 10.3390/vaccines11061043.
Vaccines play an important role in maintaining human and animal health worldwide. There is continued demand for effective and safe adjuvants capable of enhancing antigen-specific responses to a target pathogen. Rabbit hemorrhagic disease virus (RHDV) is a highly contagious calicivirus that often induces high mortality rates in rabbits. Herein, we evaluated the activity of an experimental sulfated lactosyl archaeol (SLA) archaeosome adjuvant when incorporated in subunit vaccine formulations targeting RHDV. The subunit antigens consisted of RHDV-CRM peptide conjugates or recombinant RHDV2 VP60. SLA was able to enhance antigen-specific antibody titers and cellular responses in mice and rabbits. Three weeks following immunization, antigen-specific antibody levels in rabbits vaccinated with RHDV2 VP60 + SLA were significantly higher than those immunized with antigen alone, with geomean titers of 7393 vs. 117. In addition, the SLA-adjuvanted VP60-based formulations were highly efficacious in a rabbit RHDV2 challenge model with up to 87.5% animals surviving the viral challenge. These findings demonstrate the potential utility of SLA adjuvants in veterinary applications and highlight its activity in different types of mammalian species.
疫苗在维护全球人类和动物健康方面发挥着重要作用。对能够增强针对目标病原体的抗原特异性反应的有效且安全的佐剂一直存在需求。兔出血性疾病病毒（RHDV）是一种高度传染性的杯状病毒，常导致兔子的高死亡率。在此，我们评估了一种实验性硫酸化乳糖基古菌醇（SLA）古脂质体佐剂在靶向RHDV的亚单位疫苗制剂中的活性。亚单位抗原由RHDV-CRM肽缀合物或重组RHDV2 VP60组成。SLA能够增强小鼠和兔子体内的抗原特异性抗体滴度和细胞反应。免疫三周后，用RHDV2 VP60 + SLA免疫的兔子体内的抗原特异性抗体水平显著高于仅用抗原免疫的兔子，几何平均滴度分别为7393和117。此外，基于SLA佐剂的VP60制剂在兔RHDV2攻毒模型中具有高度有效性，高达87.5%的动物在病毒攻毒中存活。这些发现证明了SLA佐剂在兽医应用中的潜在效用，并突出了其在不同类型哺乳动物物种中的活性。
