Departments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, and Barnett Institute, Northeastern University, Boston, MA 02115, USA.
Parallel Squared Technology Institute, Watertown, MA 02472, USA.
Development. 2023 Jul 1;150(13). doi: 10.1242/dev.201492. Epub 2023 Jun 30.
Many developmental processes are regulated post-transcriptionally. Such post-transcriptional regulatory mechanisms can now be analyzed by robust single-cell mass spectrometry methods that allow accurate quantification of proteins and their modification in single cells. These methods can enable quantitative exploration of protein synthesis and degradation mechanisms that contribute to developmental cell fate specification. Furthermore, they may support functional analysis of protein conformations and activities in single cells, and thus link protein functions to developmental processes. This Spotlight provides an accessible introduction to single-cell mass spectrometry methods and suggests initial biological questions that are ripe for investigation.
许多发育过程受到转录后调控。现在，通过稳健的单细胞质谱方法可以分析这些转录后调控机制，该方法能够准确定量单个细胞中的蛋白质及其修饰。这些方法可以定量探索有助于发育细胞命运特化的蛋白质合成和降解机制。此外，它们还可以支持单个细胞中蛋白质构象和活性的功能分析，并将蛋白质功能与发育过程联系起来。本特写提供了对单细胞质谱方法的简单介绍，并提出了一些适合进行研究的初始生物学问题。
