Department of Molecular Medicine, Cell and Matrix Research Institute (CMRI), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea.
Nutrients. 2023 Apr 27;15(9):2107. doi: 10.3390/nu15092107.
Osteoblasts and osteoclasts play crucial roles in bone formation and bone resorption. We found that plum-derived exosome-like nanovesicles (PENVs) suppressed osteoclast activation and modulated osteoblast differentiation. PENVs increased the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells and osteoblasts from mouse bone marrow cultures. Notably, PENVs elevated the expression of osteoblastic transcription factors and osteoblast differentiation marker proteins in MC3T3-E1 cells. Higher levels of phosphorylated BMP-2, p38, JNK, and smad1 proteins were detected in PENV-treated MC3T3-E1 cells. Additionally, the number of TRAP-positive cells was significantly decreased in PENV-treated osteoclasts isolated from osteoblasts from mouse bone marrow cultures. Importantly, osteoclastogenesis of marker proteins such as PPAR-gamma, NFATc1, and c-Fos were suppressed by treatment with PENVs (50 μg/mL). Taken together, these results demonstrate that PENVs can be used as therapeutic targets for treating bone-related diseases by improving osteoblast differentiation and inhibiting osteoclast activation for the first time.
成骨细胞和破骨细胞在骨形成和骨吸收中起着至关重要的作用。我们发现，李属衍生的外泌体样纳米囊泡（PENVs）抑制破骨细胞的激活并调节成骨细胞的分化。PENVs 增加了成骨细胞 MC3T3-E1 细胞和来自鼠骨髓培养物的成骨细胞的增殖、分化和矿化。值得注意的是，PENVs 提高了 MC3T3-E1 细胞中成骨细胞转录因子和成骨细胞分化标志物蛋白的表达。在经 PENVs 处理的 MC3T3-E1 细胞中，检测到磷酸化 BMP-2、p38、JNK 和 smad1 蛋白水平升高。此外，在经 PENVs 处理的来自鼠骨髓培养物的成骨细胞中分离的破骨细胞中，TRAP 阳性细胞的数量显著减少。重要的是，PENVs（50μg/ml）处理抑制了破骨细胞标志物蛋白如 PPAR-γ、NFATc1 和 c-Fos 的破骨细胞生成。综上所述，这些结果表明，PENVs 可首次被用作通过改善成骨细胞分化和抑制破骨细胞激活来治疗与骨相关疾病的治疗靶点。
