Kirchner Róbert, Kirchnerová Soňa, Tirpák Filip, Halo Marko, Slanina Tomáš, Tokárová Katarína, Kováčik Anton, Miškeje Michal, Komárňanská Veronika, Greń Agnieszka, Formicki Grzegorz, Massányi Peter
Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Institute of Animal Husbandry, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Animals (Basel). 2023 Jul 6;13(13):2218. doi: 10.3390/ani13132218.
Conservation of species diversity is the need of the hour for preserving life forms on Earth. Extinction of any part of the ecosystem has negative impacts on many processes and systems. The objective of this work was to analyze some biochemical and molecular indicators and their correlations to biogenic elements and heavy metals in ( = 16).
Biochemical parameters were analyzed using the commercial kit DiaSys and biochemical analyzer Randox RX Monza. Sodium, potassium, and chlorides were measured using the EasyLite analyzer. Oxidative stress was evaluated using colorimetric and luminometric methods. Quantification of chemical elements in the blood was carried out using inductively coupled plasma mass spectrometry (ICPS).
Biochemical values of analyzed samples from Hermann's tortoises were almost the same as referential values described by multiple authors, with minor aberrations in the total protein parameter. Values of arsenic (As) and nickel (Ni) showed correlation with biochemical parameters and the parameters of oxidative stress. Cadmium (Cd) exhibited correlation with aspartate aminotransferase (AST).
This study reports correlations among four heavy metals, and their levels were again correlated with biochemical and molecular parameters in Hermann's tortoises.
保护物种多样性是当下保护地球上生命形式的迫切需求。生态系统任何部分的灭绝都会对许多过程和系统产生负面影响。本研究的目的是分析16只赫尔曼陆龟的一些生化和分子指标及其与生物成因元素和重金属的相关性。
使用DiaSys商业试剂盒和Randox RX Monza生化分析仪分析生化参数。使用EasyLite分析仪测量钠、钾和氯化物。使用比色法和发光法评估氧化应激。使用电感耦合等离子体质谱法（ICPS）对血液中的化学元素进行定量分析。
赫尔曼陆龟分析样本的生化值与多位作者描述的参考值几乎相同，总蛋白参数有轻微偏差。砷（As）和镍（Ni）的值与生化参数和氧化应激参数相关。镉（Cd）与天冬氨酸转氨酶（AST）相关。
本研究报告了四种重金属之间的相关性，其水平再次与赫尔曼陆龟的生化和分子参数相关。
