Burdusel Alexandra-Cristina, Neacsu Ionela Andreea, Birca Alexandra Catalina, Chircov Cristina, Grumezescu Alexandru-Mihai, Holban Alina Maria, Curutiu Carmen, Ditu Lia Mara, Stan Miruna, Andronescu Ecaterina
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 1-7 Gheorghe Polizu Street, 011061 Bucharest, Romania.
Academy of Romanian Scientists, Splaiul Independentei 54, 050044 Bucharest, Romania.
J Funct Biomater. 2023 Jul 19;14(7):378. doi: 10.3390/jfb14070378.
Orthopedic bone graft infections are major complications in today's medicine, and the demand for antibacterial treatments is expanding because of the spread of antibiotic resistance. Various compositions of hydroxyapatite (HAp) in which Calcium (Ca) ions are substituted with Cerium (Ce) and Magnesium (Mg) are herein proposed as biomaterials for hard tissue implants. This approach gained popularity in recent years and, in the pursuit of mimicking the natural bone mineral's composition, over 70 elements of the Periodic Table were already reported as substituents into HAp structure. The current study aimed to create materials based on HAp, Hap-Ce, and Hap-Mg using hydrothermal maturation in the microwave field. This route has been considered a novel, promising, and effective way to obtain monodisperse, fine nanoparticles while easily controlling the synthesis parameters. The synthesized HAp powders were characterized morphologically and structurally by XRD diffraction, Dynamic light scattering, zeta potential, FTIR spectrometry, and SEM analysis. Proliferation and morphological analysis on osteoblast cell cultures were used to demonstrate the cytocompatibility of the produced biomaterials. The antimicrobial effect was highlighted in the synthesized samples, especially for hydroxyapatite substituted with cerium. Therefore, the samples of HAp substituted with cerium or magnesium are proposed as biomaterials with enhanced osseointegration, also having the capacity to reduce device-associated infections.
骨科骨移植感染是当今医学中的主要并发症，由于抗生素耐药性的传播，对抗菌治疗的需求正在扩大。本文提出了各种用铈（Ce）和镁（Mg）取代钙（Ca）离子的羟基磷灰石（HAp）组合物作为硬组织植入物的生物材料。近年来，这种方法受到了广泛关注，为了模仿天然骨矿物质的组成，元素周期表中的70多种元素已被报道可作为取代基引入HAp结构中。本研究旨在利用微波场中的水热成熟法制备基于HAp、Hap-Ce和Hap-Mg的材料。这种方法被认为是一种新颖、有前景且有效的方法，可获得单分散的细纳米颗粒，同时易于控制合成参数。通过XRD衍射、动态光散射、zeta电位、FTIR光谱和SEM分析对合成的HAp粉末进行了形态和结构表征。通过对成骨细胞培养物的增殖和形态分析来证明所制备生物材料的细胞相容性。合成样品中突出显示了抗菌效果，尤其是铈取代的羟基磷灰石。因此，铈或镁取代的HAp样品被提议作为具有增强骨整合能力的生物材料，同时也具有减少与器械相关感染的能力。
