Possati L, Collesi A, Maracchini G, Muzzonigro G, Polito M
Urol Res. 1986;14(3):133-6. doi: 10.1007/BF00255831.
The transforming activity of DNA extracted from one testicular cancer, one benign prostatic hyperplasia, two malignant prostatic adenocarcinomas, six renal cell carcinomas, two tumours of the renal pelvis, a specimen of normal urothelial tissue, two benign papillomas of the bladder and five malignant bladder carcinomas, was investigated using a DNA transfection assay on primary cultures of hamster kidney cells (HK cells) and mouse embryo fibroblasts (MEF cells). Transformation was scored under light microscope by the appearance of foci of morphologically transformed cells and by evaluating the ability of the transfected cells to from colonies in soft agar. Primary HK cells were unable to support transformation by human tumour DNA because of their very limited lifespan in vitro; MEF cultures, on the contrary, had a much more prolonged life: but were only abortively transformed by DNA from four out of six renal cell carcinomas and four out of seven malignant urothelial tumours, suggesting that transforming genes are present but not activated in the DNA from these urological malignancies.
利用仓鼠肾细胞（HK细胞）和小鼠胚胎成纤维细胞（MEF细胞）的原代培养物进行DNA转染试验，研究了从一例睾丸癌、一例良性前列腺增生、两例恶性前列腺腺癌、六例肾细胞癌、两例肾盂肿瘤、一份正常尿路上皮组织标本、两例膀胱良性乳头状瘤和五例恶性膀胱癌中提取的DNA的转化活性。在光学显微镜下，通过形态转化细胞集落的出现以及评估转染细胞在软琼脂中形成集落的能力来对转化进行评分。原代HK细胞由于其在体外的寿命非常有限，无法支持人肿瘤DNA的转化；相反，MEF培养物的寿命要长得多，但仅被六例肾细胞癌中的四例以及七例恶性尿路上皮肿瘤中的四例的DNA进行了流产性转化，这表明在这些泌尿系统恶性肿瘤的DNA中存在转化基因，但未被激活。
