Nature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 21, BG-1113 Sofia, Bulgaria.
Int J Mol Sci. 2023 Jul 13;24(14):11402. doi: 10.3390/ijms241411402.
Cold stress is among the most important environmental factors reducing the yield of crops. The present study aimed to investigate the impact of increasing cold stress conditions on winter oilseed rape polyamines, proline, and ethylene metabolism in acclimated and non-acclimated winter oilseed rape. This study was carried out under controlled conditions in the laboratory. The winter oilseed rape hybrid 'Visby' was used in the experiment. Acclimated and non-acclimated plants were subjected to a two-day-long increasing cold (from -1 °C to -3 °C) treatment. HPTLC, RT-qPCR, spectral analysis, and gas chromatography methods were used to analyse the levels of polyamines, gene expression, proline, and ethylene, respectively. This study showed a decrease in putrescine, spermidine, and spermine content during cold acclimation and a decrease in putrescine and spermidine levels at sub-zero temperatures. There were intensive changes in ADC2 gene expression, proline, and ethylene levels in non-acclimated plants: a substantial increase after exposure to -1 °C temperature and a sharp decrease after exposure to -3 °C temperature. The changes in these parameters were lower or absent in acclimated plants. The phenomena observed in this study add new insights to the knowledge about the plant stress response and suggest questions to be answered in the future.
冷胁迫是降低作物产量的最重要环境因素之一。本研究旨在探讨在适应和不适应的冬季油菜中，增加冷胁迫条件对多胺、脯氨酸和乙烯代谢的影响。本研究在实验室控制条件下进行。实验采用冬季油菜杂交种‘Visby’。适应和不适应的植物分别接受为期两天的逐渐降温（从-1°C 到-3°C）处理。使用 HPTLC、RT-qPCR、光谱分析和气相色谱法分别分析多胺、基因表达、脯氨酸和乙烯的水平。研究表明，在冷驯化过程中腐胺、亚精胺和精胺含量降低，在亚零温度下腐胺和亚精胺水平降低。非适应植物中 ADC2 基因表达、脯氨酸和乙烯水平发生剧烈变化：在暴露于-1°C 温度后显著增加，在暴露于-3°C 温度后急剧下降。适应植物中的这些参数变化较低或不存在。本研究中观察到的现象为植物应激反应的知识增添了新的见解，并提出了未来需要回答的问题。
