Karki Anand B, Khatri Bhuwan, Fakhr Mohamed K
Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA.
Genes and Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Pathogens. 2023 Jul 21;12(7):960. doi: 10.3390/pathogens12070960.
spp. are known to cause campylobacteriosis, a bacterial disease that remains a public health threat. spp. are prevalent in retail meat and liver products, and the prolonged survival of in the low temperatures needed for storage is a challenge for food safety. In this study, RNA-seq was used for the analysis of the HC2-48 (Cc48) and OD2-67 (Cj67) transcriptomes at 4 °C in a nutrient-rich medium (chicken juice, CJ) and Mueller-Hinton broth (MHB) for 0 h, 0.5 h, 24 h and 48 h. Differentially expressed genes (DEGs) involved in flagellar assembly were highly impacted by low temperatures (4 °C) in HC2-48, whereas genes related to the ribosome and ribonucleoprotein complex were modulated for OD2-67 at 4 °C. Most of the DEGs in cells grown at 4 °C in the two medium formulations were not significantly expressed at different incubation times. Although more DEGs were observed in CJ as compared to MHB in both strains, the absence of common genes expressed at all incubation times indicates that the food matrix environment is not the sole determinant of differential expression in spp. at low temperatures.
已知某些弯曲杆菌属物种会引发弯曲杆菌病，这是一种仍然对公共卫生构成威胁的细菌性疾病。弯曲杆菌属物种在零售肉类和肝脏产品中普遍存在，并且它们在储存所需的低温环境下能长时间存活，这对食品安全构成了挑战。在本研究中，利用RNA测序分析了HC2 - 48（Cc48）和OD2 - 67（Cj67）在富含营养的培养基（鸡肉汁，CJ）和Mueller - Hinton肉汤（MHB）中于4℃培养0小时、0.5小时、24小时和48小时后的转录组。参与鞭毛组装的差异表达基因（DEG）在HC2 - 48中受低温（4℃）影响很大，而与核糖体和核糖核蛋白复合物相关的基因在4℃时对OD2 - 67有调控作用。在两种培养基配方中于4℃培养的细胞中，大多数差异表达基因在不同孵育时间并未显著表达。尽管在两种菌株中，与MHB相比，在CJ中观察到更多差异表达基因，但在所有孵育时间均未发现共同表达的基因，这表明食品基质环境并非弯曲杆菌属物种在低温下差异表达的唯一决定因素。
