Transcriptome Analysis of and during Cold Stress.

spp. are known to cause campylobacteriosis, a bacterial disease that remains a public health threat. spp. are prevalent in retail meat and liver products, and the prolonged survival of in the low temperatures needed for storage is a challenge for food safety. In this study, RNA-seq was used for the analysis of the HC2-48 (Cc48) and OD2-67 (Cj67) transcriptomes at 4 °C in a nutrient-rich medium (chicken juice, CJ) and Mueller-Hinton broth (MHB) for 0 h, 0.5 h, 24 h and 48 h. Differentially expressed genes (DEGs) involved in flagellar assembly were highly impacted by low temperatures (4 °C) in HC2-48, whereas genes related to the ribosome and ribonucleoprotein complex were modulated for OD2-67 at 4 °C. Most of the DEGs in cells grown at 4 °C in the two medium formulations were not significantly expressed at different incubation times. Although more DEGs were observed in CJ as compared to MHB in both strains, the absence of common genes expressed at all incubation times indicates that the food matrix environment is not the sole determinant of differential expression in spp. at low temperatures.