Ugalde-Arbizu Maider, Aguilera-Correa John Jairo, San Sebastian Eider, Páez Paulina L, Nogales Estela, Esteban Jaime, Gómez-Ruiz Santiago
Departamento de Química Aplicada, Facultad de Química, Euskal Herriko Unibertsitatea (UPV/EHU), Paseo Manuel Lardizabal 3, 20018 San Sebastián, Spain.
Clinical Microbiology Department, IIS-Fundación Jiménez Diaz, UAM, Avenida Reyes Católicos 2, 28040 Madrid, Spain.
Pharmaceuticals (Basel). 2023 Jul 5;16(7):961. doi: 10.3390/ph16070961.
Antibiotic resistance is a global problem and bacterial biofilms contribute to its development. In this context, this study aimed to perform the synthesis and characterization of seven materials based on silica mesoporous nanoparticles functionalized with three types of fluoroquinolones, along with Cu or Ag species to evaluate the antibacterial properties against , , , and , including clinical and multi-drug-resistant strains of and . In addition, in order to obtain an effective material to promote wound healing, a well-known proliferative agent, phenytoin sodium, was adsorbed onto one of the silver-functionalized materials. Furthermore, biofilm studies and the generation of reactive oxygen species (ROS) were also carried out to determine the antibacterial potential of the synthesized materials. In this sense, the Cu materials showed antibacterial activity against and , potentially due to increased ROS generation (up to 3 times), whereas the Ag materials exhibited a broader spectrum of activity, even inhibiting clinical strains of MRSA and In particular, the Ag material with phenytoin sodium showed the ability to reduce biofilm development by up to 55% and inhibit bacterial growth in a "wound-like medium" by up to 89.33%.
抗生素耐药性是一个全球性问题，细菌生物膜在其发展过程中起到了推动作用。在此背景下，本研究旨在合成并表征七种基于介孔二氧化硅纳米颗粒的材料，这些纳米颗粒用三种类型的氟喹诺酮以及铜或银物种进行了功能化修饰，以评估其对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和鲍曼不动杆菌的抗菌性能，包括这些细菌的临床菌株和多重耐药菌株。此外，为了获得一种促进伤口愈合的有效材料，将一种知名的增殖剂苯妥英钠吸附到其中一种银功能化材料上。此外，还进行了生物膜研究和活性氧（ROS）生成实验，以确定合成材料的抗菌潜力。从这个意义上说，铜材料对金黄色葡萄球菌和大肠杆菌表现出抗菌活性，这可能是由于ROS生成增加（高达3倍），而银材料表现出更广泛的活性谱，甚至能抑制耐甲氧西林金黄色葡萄球菌的临床菌株。特别是，含有苯妥英钠的银材料显示出能够将生物膜形成减少多达55%，并在“伤口样培养基”中抑制细菌生长多达89.33%。
