Lai Wei-Yi, Feng Sheng-Wei, Chan Ya-Hui, Chang Wei-Jen, Wang Hsin-Ta, Huang Haw-Ming
School of Organic and Polymeric, National Taipei University of Technology, Taipei 10608, Taiwan.
School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan.
Polymers (Basel). 2018 Jul 22;10(7):804. doi: 10.3390/polym10070804.
Fe₃O₄ nanoparticles were loaded into poly-l-lactide (PLLA) with concentrations of 2% and 5%, respectively, using an electrospinning method. In vivo animal experiments were then performed to evaluate the potential of the Fe₃O₄/PLLA nanofibrous material for bone tissue engineering applications. Bony defects with a diameter of 4 mm were prepared in rabbit tibias. Fe₃O₄/PLLA nanofibers were grafted into the drilled defects and histological examination and computed tomography (CT) image detection were performed after an eight-week healing period. The histological results showed that the artificial bony defects grafted with Fe₃O₄/PLLA nanofibers exhibited a visibly higher bone healing activity than those grafted with neat PLLA. In addition, the quantitative results from CT images revealed that the bony defects grafted with 2% and 5% Fe₃O₄/PLLA nanofibers, respectively, showed 1.9- and 2.3-fold increases in bone volume compared to the control blank sample. Overall, the results suggest that the Fe₃O₄/PLLA nanofibers fabricated in this study may serve as a useful biomaterial for future bone tissue engineering applications.
采用静电纺丝法将四氧化三铁纳米颗粒分别以2%和5%的浓度载入聚左旋乳酸（PLLA）中。随后进行体内动物实验，以评估四氧化三铁/聚左旋乳酸纳米纤维材料在骨组织工程应用中的潜力。在兔胫骨上制备直径为4毫米的骨缺损。将四氧化三铁/聚左旋乳酸纳米纤维植入钻孔缺损处，在八周愈合期后进行组织学检查和计算机断层扫描（CT）图像检测。组织学结果表明，与单纯聚左旋乳酸植入的骨缺损相比，植入四氧化三铁/聚左旋乳酸纳米纤维的人工骨缺损表现出明显更高的骨愈合活性。此外，CT图像的定量结果显示，分别植入2%和5%四氧化三铁/聚左旋乳酸纳米纤维的骨缺损与对照空白样本相比，骨体积分别增加了1.9倍和2.3倍。总体而言，结果表明本研究制备的四氧化三铁/聚左旋乳酸纳米纤维可作为未来骨组织工程应用的有用生物材料。
