Chen Yilin, Li Wenwen, Jia Kai, Liao Kang, Liu Liqiang, Fan Guoquan, Zhang Shikui, Wang Yatong
College of Horticulture, Xinjiang Agricultural University, Urumqi, China.
Postdoctoral Research Station of Crop Science, Xinjiang Agricultural University, Urumqi, China.
Front Plant Sci. 2023 Jul 18;14:1210309. doi: 10.3389/fpls.2023.1210309. eCollection 2023.
Flavonoids, as secondary metabolites in plants, play important roles in many biological processes and responses to environmental factors.
Apricot fruits are rich in flavonoid compounds, and in this study, we performed a combined metabolomic and transcriptomic analysis of orange flesh (JN) and white flesh (ZS) apricot fruits.
A total of 222 differentially accumulated flavonoids (DAFs) and 15855 differentially expressed genes (DEGs) involved in flavonoid biosynthesis were identified. The biosynthesis of flavonoids in apricot fruit may be regulated by 17 enzyme-encoding genes, namely PAL (2), 4CL (9), C4H (1), HCT (15), C3'H (4), CHS (2), CHI (3), F3H (1), F3'H (CYP75B1) (2), F3'5'H (4), DFR (4), LAR (1), FLS (3), ANS (9), ANR (2), UGT79B1 (6) and CYP81E (2). A structural gene-transcription factor (TF) correlation analysis yielded 3 TFs (2 bHLH, 1 MYB) highly correlated with 2 structural genes. In addition, we obtained 26 candidate genes involved in the biosynthesis of 8 differentially accumulated flavonoids metabolites in ZS by weighted gene coexpression network analysis. The candidate genes and transcription factors identified in this study will provide a highly valuable molecular basis for the in-depth study of flavonoid biosynthesis in apricot fruits.
类黄酮作为植物中的次生代谢产物，在许多生物过程和对环境因素的响应中发挥着重要作用。
杏果实富含类黄酮化合物，在本研究中，我们对橙色果肉（JN）和白色果肉（ZS）杏果实进行了代谢组学和转录组学的联合分析。
共鉴定出222种差异积累的类黄酮（DAF）和15855个参与类黄酮生物合成的差异表达基因（DEG）。杏果实中类黄酮的生物合成可能受17个编码酶的基因调控，即苯丙氨酸解氨酶（PAL）（2个）、4-香豆酸辅酶A连接酶（4CL）（9个）、肉桂酸4-羟化酶（C4H）（1个）、对香豆酸3-羟化酶（HCT）（15个）、咖啡酸3-甲基转移酶（C3'H）（4个）、查尔酮合酶（CHS）（2个）、查尔酮异构酶（CHI）（3个）、黄烷酮3-羟化酶（F3H）（1个）、黄酮醇3'-羟化酶（F3'H）（细胞色素P450 75B1）（2个）、黄酮醇3'5'-羟化酶（F3'5'H）（4个）、二氢黄酮醇4-还原酶（DFR）（4个）、无色花青素还原酶（LAR）（1个）、黄酮醇合成酶（FLS）（3个）、花青素合成酶（ANS）（9个）、花青素还原酶（ANR）（2个）、UDP-葡萄糖：类黄酮-7-O-葡萄糖基转移酶（UGT79B1）（6个）和细胞色素P450 81E（2个）。通过结构基因-转录因子（TF）相关性分析，得到3个与2个结构基因高度相关的转录因子（2个bHLH，1个MYB）。此外，通过加权基因共表达网络分析，我们获得了26个参与ZS中8种差异积累的类黄酮代谢物生物合成的候选基因。本研究中鉴定出的候选基因和转录因子将为深入研究杏果实中类黄酮的生物合成提供极具价值的分子基础。
