Ahmed Nasim, Chandra Dey Shaikat, Mustary Nusrat, Ashaduzzaman Md
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering and Technology, University of Dhaka, Dhaka, 1000, Bangladesh.
Institute of Fuel Research and Development (IFRD), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka, 1205, Bangladesh.
Heliyon. 2023 Jul 16;9(8):e18153. doi: 10.1016/j.heliyon.2023.e18153. eCollection 2023 Aug.
Nanostructured metal oxide particles with diversified morphologies are in high demand in nanotechnology. The particle size, shape, and overall geometry mainly depend on the fabrication method. This study reports synthesis of zinc oxide nanoparticles (ZnO NPs) from zinc nitrate hexahydrate [Zn(NO).6HO] precursor in aqueous media at 65 °C by using lactose from cow milk as a reducing agent and regulating pH from 6 to 10. UV-visible absorption gave maximum absorbance () at 371-375 nm in ethanol for localized surface plasmon resonance (LSPR), FTIR exhibited bands at ca. 439-481 cm for stretching mode Zn-O bonds, and XRD peaks at 2 values at 31.8, 34.45, and 36.28° confirmed the fabricated ZnO NPs. The XRD spectra also indicated that the ZnO crystallite (20-30 nm) has a hexagonal wurtzite structure. The average particle sizes measured by DLS were ca. 50-837 nm, and SEM microphotographs demonstrated the morphology of ZnO NPs with a hexagonal, rod-shaped, or spike-like structure. The ZnO NPs were used to investigate the LSPR absorption at various concentrations of insulin, ranging from 2.5 μM to 50 μM. The ZnO NPs fabricated at pH 7 and 10 showed better insulin sensing performance with high precision. The synthesis approach of ZnO NPs with variable morphologies would play a significant function in biomedical science especially real time monitoring of glucose for efficient management of diabetes.
纳米技术对具有多种形态的纳米结构金属氧化物颗粒有很高的需求。颗粒的大小、形状和整体几何形状主要取决于制造方法。本研究报告了在65°C的水介质中，以牛奶中的乳糖作为还原剂，将pH值从6调节到10，由六水合硝酸锌[Zn(NO₃)₂·6H₂O]前驱体制备氧化锌纳米颗粒（ZnO NPs）。紫外可见吸收光谱在乙醇中371 - 375 nm处出现最大吸光度（λmax），用于局域表面等离子体共振（LSPR）；傅里叶变换红外光谱在约439 - 481 cm⁻¹处出现Zn - O键拉伸模式的谱带；X射线衍射在2θ值为31.8°、34.45°和36.28°处出现峰，证实了制备的ZnO NPs。XRD光谱还表明，ZnO微晶（20 - 30 nm）具有六方纤锌矿结构。通过动态光散射测量的平均粒径约为50 - 837 nm，扫描电子显微镜照片展示了具有六边形、棒状或尖状结构的ZnO NPs的形态。使用ZnO NPs研究了在2.5 μM至50 μM的各种胰岛素浓度下的LSPR吸收。在pH值为7和10时制备的ZnO NPs表现出更好的胰岛素传感性能，具有高精度。具有可变形态的ZnO NPs的合成方法将在生物医学科学中发挥重要作用，特别是在糖尿病的有效管理中对葡萄糖的实时监测。
