Niu Qing, Wang Xiaoxu, Qi Xinyong, Cao Changjian, Yang Kaixuan, Gu Caiju, Zhou Zhenxiang, Huang Qizhong
Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Science, Shanghai, China.
Shanghai Animal Disease Control Center, Shanghai, China.
Front Microbiol. 2023 Jul 27;14:1216542. doi: 10.3389/fmicb.2023.1216542. eCollection 2023.
Pullorum disease is one of the common bacterial infectious diseases caused by (), which can result in a decrease in the reproductive performance of laying hens, thus causing considerable economic losses. However, studies about the characteristics of intestinal microbiota with pullorum and their potential association with reproductive performance in hens are still limited. This study was to identify the gut microbiota associated with in poultry.
A total of 30 hens with -negative (PN) and 30 hens with -positive (PP) were analyzed for hatching eggs laid in 2 weeks (HEL), fertilization eggs (FE), chick number (CN), and microbial structure.
There were significant differences in HEL ( < 0.01), FE ( < 0.01), and CN ( < 0.01) between PP and PN. Histomorphological observations showed abnormal morphology of the ovaries and fallopian tubes and low integrity of epithelial tissue in the ileum and cecum in PP. 16S rRNA gene sequencing revealed that beneficial cecal microbes, such as , and , were positively correlated with reproductive performance and had lower abundance in PP ( = 0.001). Furthermore, diminished phosphotransferase system (PTS) and pentose phosphate pathway, butanoate metabolism and oxidative phosphorylation were also found in PP.
Taken together, this study clarified the morphological characteristics of the reproductive tract and intestines of chickens infected with and preliminarily explored the potential association between cecal microbiota and reproductive performance in hens. Our data may provide a reference for revealing the intestinal microbial characteristics of hens in resisting pullorum and exploring novel approaches to infection control in future studies.
鸡白痢是由（）引起的常见细菌性传染病之一，可导致蛋鸡繁殖性能下降，从而造成相当大的经济损失。然而，关于感染鸡白痢的母鸡肠道微生物群特征及其与繁殖性能的潜在关联的研究仍然有限。本研究旨在鉴定家禽中与鸡白痢相关的肠道微生物群。
对30只鸡白痢阴性（PN）母鸡和30只鸡白痢阳性（PP）母鸡的2周内产的孵化蛋（HEL）、受精卵（FE）、雏鸡数量（CN）和微生物结构进行分析。
PP组和PN组在HEL（<0.01）、FE（<0.01）和CN（<0.01）方面存在显著差异。组织形态学观察显示，PP组卵巢和输卵管形态异常，回肠和盲肠上皮组织完整性低。16S rRNA基因测序显示，有益的盲肠微生物，如（），与繁殖性能呈正相关，且在PP组中丰度较低（=0.001）。此外，PP组还发现磷酸转移酶系统（PTS）和磷酸戊糖途径、丁酸代谢和氧化磷酸化减少。
综上所述，本研究阐明了感染鸡白痢的鸡的生殖道和肠道的形态特征，并初步探讨了盲肠微生物群与母鸡繁殖性能之间的潜在关联。我们的数据可能为未来研究揭示母鸡抵抗鸡白痢的肠道微生物特征和探索新的感染控制方法提供参考。
