Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Elanco Animal Health, Mutiara Damansara, Selangor, Malaysia.
J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skad257.
We investigated the effects of different Bacillus subtilis QST713 doses and a B. subtilis QST713 and β-mannanase mix on growth performance, intestinal barrier function, and gut microbiota in weaned piglets. In total, 320 healthy piglets were randomly assigned to four groups: 1) control group (basal diet), 2) BS100 group (basal diet plus 100 mg/kg B. subtilis QST713), 3) BS200 group (basal diet plus 200 mg/kg B. subtilis QST713), and 4) a BS100XT group (basal diet plus 100 mg/kg B. subtilis QST713 and 150 mg/kg β-mannanase). The study duration was 42 d. We showed that feed intake in weaned piglets on days 1 to 21 was increased in group BS100 (P < 0.05), and that the feed conversion ratio in group BS100XT animals decreased throughout the study (P < 0.05). In terms of microbial counts, the BS100XT group showed reduced Escherichia coli and Clostridium perfringens numbers on day 21 (P < 0.05). Moreover, no significant α-diversity differences were observed across all groups during the study (P > 0.05). However, principal coordinates analysis indicated clear separations in bacterial community structures across groups (analysis of similarities: P < 0.05) on days 21 and 42. Additionally, E-cadherin, occludin, and zonula occludens-1 (ZO-1) expression in piglet feces increased (P < 0.05) by adding B. subtilis QST713 and β-mannanase to diets. Notably, this addition decreased short-chain fatty acid concentrations. In conclusion, B. subtilis QST713 addition or combined B. subtilis QST713 plus β-mannanase effectively improved growth performance, intestinal barrier function, and microbial balance in weaned piglets.
我们研究了不同枯草芽孢杆菌 QST713 剂量和枯草芽孢杆菌 QST713 与β-甘露聚糖酶混合物对断奶仔猪生长性能、肠道屏障功能和肠道微生物群的影响。总共将 320 头健康仔猪随机分为四组：1）对照组（基础日粮），2）BS100 组（基础日粮加 100mg/kg 枯草芽孢杆菌 QST713），3）BS200 组（基础日粮加 200mg/kg 枯草芽孢杆菌 QST713），和 4）BS100XT 组（基础日粮加 100mg/kg 枯草芽孢杆菌 QST713 和 150mg/kgβ-甘露聚糖酶）。研究持续 42 天。我们发现，在第 1 至 21 天，BS100 组断奶仔猪的采食量增加（P＜0.05），而 BS100XT 组的饲料转化率在整个研究过程中降低（P＜0.05）。就微生物计数而言，BS100XT 组在第 21 天降低了大肠杆菌和产气荚膜梭菌的数量（P＜0.05）。此外，在整个研究过程中，各组之间的α多样性差异均无统计学意义（P＞0.05）。然而，主坐标分析表明，在第 21 天和第 42 天，各组的细菌群落结构明显分离（相似性分析：P＜0.05）。此外，添加枯草芽孢杆菌 QST713 和β-甘露聚糖酶可增加仔猪粪便中 E-钙粘蛋白、闭合蛋白和封闭蛋白-1（ZO-1）的表达（P＜0.05）。值得注意的是，这种添加降低了短链脂肪酸的浓度。综上所述，添加枯草芽孢杆菌 QST713 或联合添加枯草芽孢杆菌 QST713 加β-甘露聚糖酶可有效改善断奶仔猪的生长性能、肠道屏障功能和微生物平衡。
