Liu Yisi, Gu Wei, Liu Xiaoyi, Zou Youwei, Wu Yujun, Xu Youhan, Han Dandan, Wang Junjun, Zhao Jinbiao
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Shandong Provincial Key Laboratory of Animal Microecological Agent, Shandong Baolai Leelai Bioengineering Co., Ltd., Tai'an 271000, China.
Vet Sci. 2022 Nov 30;9(12):668. doi: 10.3390/vetsci9120668.
This study was conducted to explore the effects of the joint application of Lactobacillus plantarum and Bacillus subtilis on growth performance, immune function, antioxidant capacity, intestinal integrity, and gut microbiota composition in weaned piglets. The piglets were allocated randomly into 4 dietary groups, which were a control diet (NC), NC + 150 ppm mucilage sulfate (PC), and 3 additional diets containing 1 kg/t (LT), 1.5 kg/t (MT), or 2 kg/t (HT) mixture of Lactobacillus plantarum and Bacillus subtilis, respectively. Results showed that joint application of Lactobacillus plantarum and Bacillus subtilis increased ADFI and ADG of weaned piglets in d 1428 and d 2842 (p < 0.05), and decreased serum concentrations of DAO, IL-1β, TNF-α, and IL-2. The LT group increased jejunal and colonic sIgA contents compared with the PC group (p < 0.05). Groups of MT and HT increased colonic mRNA expression of host defense peptides and tight junction proteins compared with the NC and PC groups. The joint application of Lactobacillus plantarum and Bacillus subtilis increased the abundance of colonic Lactobacillus compared with NC and PC groups (p < 0.10). In conclusion, the joint application of Lactobacillus plantarum and Bacillus subtilis as an antibiotics alternative improved growth performance via promoting immune function and intestinal integrity of weaned piglets.
本研究旨在探讨植物乳杆菌和枯草芽孢杆菌联合应用对断奶仔猪生长性能、免疫功能、抗氧化能力、肠道完整性及肠道微生物群组成的影响。仔猪被随机分为4个日粮组，分别为对照日粮（NC）、NC + 150 ppm硫酸粘杆菌素（PC），以及另外3种分别含有1 kg/t（LT）、1.5 kg/t（MT）或2 kg/t（HT）植物乳杆菌和枯草芽孢杆菌混合物的日粮。结果表明，植物乳杆菌和枯草芽孢杆菌联合应用可提高断奶仔猪在第1428天和第2842天的平均日采食量（ADFI）和平均日增重（ADG）（p < 0.05），并降低血清中D-乳酸（DAO）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和白细胞介素-2的浓度。与PC组相比，LT组空肠和结肠分泌型免疫球蛋白A（sIgA）含量增加（p < 0.05）。与NC组和PC组相比，MT组和HT组结肠中宿主防御肽和紧密连接蛋白的mRNA表达增加。与NC组和PC组相比，植物乳杆菌和枯草芽孢杆菌联合应用可增加结肠中乳酸杆菌的丰度（p < 0.10）。总之，植物乳杆菌和枯草芽孢杆菌联合应用作为抗生素替代品，通过促进断奶仔猪的免疫功能和肠道完整性来提高生长性能。
