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人类脑类器官移植到大鼠视皮层以模拟神经修复的方案。

Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair.

机构信息

Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102470. doi: 10.1016/j.xpro.2023.102470. Epub 2023 Aug 15.

DOI:10.1016/j.xpro.2023.102470
PMID:37585295
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10436235/
Abstract

Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair. For complete details on the use and execution of this protocol, please refer to Jgamadze et al..

摘要

人源干细胞衍生类器官是一种很有前途的基于移植的神经修复底物。本文描述了将前脑类器官移植到成年大鼠损伤视觉皮层中的方法。该方案包括开颅、抽吸损伤、类器官移植和颅骨修复的手术细节。该平台代表了一种有价值的工具，可用于研究类器官作为神经修复结构移植物的功效。如需了解本方案的使用和实施的详细信息，请参考 Jgamadze 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/57c4e971dd78/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/21d01d7a91a0/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/f07dd87c08b2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/9d729b7203fe/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/4d0813fd7a68/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/e5b1fe4e353b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/0606f914a006/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/16a5a72c7140/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/bb6e1daf3536/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/57c4e971dd78/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/21d01d7a91a0/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/f07dd87c08b2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/9d729b7203fe/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/4d0813fd7a68/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/e5b1fe4e353b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/0606f914a006/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/16a5a72c7140/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/bb6e1daf3536/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abaa/10436235/57c4e971dd78/gr8.jpg

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