Department of Textile Engineering, Chemistry, and Science, Wilson College of Textiles, North Carolina State University, Raleigh, NC, USA.
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Transl Vis Sci Technol. 2023 Aug 1;12(8):15. doi: 10.1167/tvst.12.8.15.
The objective of this study is to develop and characterize electrospun corneal bandage infused with Noggin protein and evaluate its therapeutic potential in the treatment of superficial nonhealing corneal ulceration.
Electrospun nanofibrous scaffolds were created with different blend ratios of polycaprolactone and gelatin and coated with different concentrations of Noggin protein. Morphologic, mechanical, degradation, and surface chemistry of the developed scaffold was assessed. Biocompatibility of the developed scaffold with corneal epithelial cells was evaluated by looking at cell viability, proliferation, and immunostaining. In vitro wound healing in the presence of Noggin-coated scaffold was evaluated by measuring wound closure rate after scratch.
Uniform nanofibrous scaffolds coated with Noggin were constructed through optimization of electrospinning parameters and demonstrated mechanical properties better than or similar to commercially available contact lenses used in corneal wound healing. In the presence of Noggin-coated scaffold, corneal epithelial cells showed higher proliferation and wound-healing rate.
This Noggin-coated electrospun scaffold represents a step toward, expanding treatment options for patients with indolent corneal ulcers.
In this study, the feasibility of Noggin-coated electrospun scaffold as a therapeutic for indolent corneal ulcer was evaluated. This study also provides a better perspective for understanding electrospun scaffolds as a tunable platform to infuse topical therapeutics and use as a corneal bandage.
本研究旨在开发并表征包埋 Noggin 蛋白的电纺角膜绷带，并评估其在治疗浅层非愈合性角膜溃疡中的治疗潜力。
采用不同比例的聚己内酯和明胶制备电纺纳米纤维支架，并涂覆不同浓度的 Noggin 蛋白。评估了所开发支架的形态、力学、降解和表面化学性能。通过观察细胞活力、增殖和免疫染色评估了所开发支架与角膜上皮细胞的生物相容性。通过测量划痕后伤口闭合率评估了 Noggin 涂层支架存在时的体外伤口愈合情况。
通过优化电纺参数构建了涂覆 Noggin 的均匀纳米纤维支架，其力学性能优于或类似于用于角膜伤口愈合的市售隐形眼镜。在 Noggin 涂层支架存在的情况下，角膜上皮细胞表现出更高的增殖和伤口愈合率。
这种包埋 Noggin 的电纺支架为治疗慢性角膜溃疡的患者提供了更多的治疗选择。
“PURPOSE”和“CONCLUSIONS”分别为首段和尾段的标题，无需翻译。
“Objective”和“Method”等大写的词汇首字母需要大写，在翻译时需保留。
“Noggin”是一种蛋白质，在本文中特指“骨形态发生蛋白抑制剂”，为避免混淆，可将其翻译为“骨形态发生蛋白抑制剂”。
原文中的 “polycaprolactone”和 “gelatin”都是化学物质，可分别翻译为“聚己内酯”和“明胶”。
原文中的 “scaffold”在不同语境下可能有不同的翻译，在此处指“支架”。
