Research Center of Protection and Utilization of Plant Resources, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
J Agric Food Chem. 2023 Aug 30;71(34):12730-12740. doi: 10.1021/acs.jafc.3c03460. Epub 2023 Aug 21.
In this study, two tigliane diterpenoids, 12-deoxyphorbol-13-hexadecanoate and 12-deoxyphorbol-13-acetate (prostratin), were identified from the methanol extract of the roots of and were found to have the ability to significantly reduce the survival of . It was determined that exposure to these two compounds had toxic effects on the growth, reproduction, locomotion behavior, and accumulation of lipids and lipofuscin of the nematodes. Moreover, the transcription levels of the genes associated with lipid accumulation, apoptosis, insulin, and nuclear hormone synthesis in were significantly influenced. Interestingly, 12-deoxyphorbol-13-hexadecanoate produced exposure toxicity at lower concentrations than that of prostratin. Pearson correlation analysis indicates that the elevated exposure toxicity of 12-deoxyphorbol-13-hexadecanoate may be the result of differing transcription levels, which result from the differential expression of , , and . These results reveal that esterification with a long-chain fatty acid elevates the exposure toxicity of this tigliane diterpenoid, thus providing a basis for the application of tigliane diterpenoids in plant-derived nematicides.
在这项研究中，从 根的甲醇提取物中鉴定出两种 型二萜，12-去氧佛波醇-13-十六烷酸酯和 12-去氧佛波醇-13-乙酸酯（普罗他汀），并发现它们具有显著降低 的生存能力。确定这两种化合物对 的生长、繁殖、运动行为以及脂质和脂褐素的积累具有毒性作用。此外，与脂质积累、细胞凋亡、胰岛素和核激素合成相关的基因的转录水平也受到显著影响。有趣的是，12-去氧佛波醇-13-十六烷酸酯在较低浓度下产生的暴露毒性比普罗他汀高。皮尔逊相关性分析表明，12-去氧佛波醇-13-十六烷酸酯暴露毒性的升高可能是由于 、 、 和 的转录水平不同导致的，这些基因的表达水平不同。这些结果表明，与长链脂肪酸的酯化作用提高了这种 型二萜的暴露毒性，从而为 型二萜在植物源杀线虫剂中的应用提供了依据。
