瑞特瑞尔三萜烯类化合物抗 HIV 活性。
Anti-HIV tigliane diterpenoids from Reutealis trisperma.
机构信息
School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, 201203, People's Republic of China; Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, 27599-7568, United States.
School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, 201203, People's Republic of China.
出版信息
Phytochemistry. 2020 Jun;174:112360. doi: 10.1016/j.phytochem.2020.112360. Epub 2020 Mar 28.
Bioassay-guided fractionation of the n-butanol extract from the branches and leaves of Reutealis trisperma resulted in the isolation of six undescribed (crotignoids L ~ Q) together with two known (12-deoxyphorbol-13-hexadecanoate and 12-deoxyphorbol-13-myristate) tigliane diterpenoids. Their structures, especially the absolute configurations, were determined from extensive spectroscopic studies, including 2D NMR spectra, CD data analysis and electronic circular dichroism (ECD) calculations. All isolates were tested for anti-HIV activity against HL4-3 virus in MT4 cells. Except for crotignoid Q, the remaining seven tigliane diterpenoids exhibited potent anti-HIV activity with IC values ranging from 0.0023 to 4.03 μM.
生物测定指导下，从三蕊柳的枝叶的正丁醇提取物中分离得到了六个未知的（crotignoids L-Q）化合物，以及两个已知的（12-去氧佛波醇-13-十六酸酯和 12-去氧佛波醇-13-肉豆蔻酸酯）替告二萜。通过广泛的光谱研究，包括二维 NMR 谱、CD 数据分析和电子圆二色性（ECD）计算，确定了它们的结构，特别是绝对构型。所有分离得到的化合物都在 MT4 细胞中针对 HL4-3 病毒进行了抗 HIV 活性测试。除了 crotignoid Q 之外，其余七个替告二萜化合物都表现出了很强的抗 HIV 活性，IC 值范围在 0.0023 到 4.03 μM 之间。
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