Smith Brooke L, King Maria D
Aerosol Technology Laboratory, Biological & Agricultural Engineering Department, Texas A&M University, College Station, TX 77843, USA.
Microorganisms. 2023 Aug 11;11(8):2068. doi: 10.3390/microorganisms11082068.
Two poultry Confined Animal Feeding Units (CAFUs), "House A" and "House B", were selected from the TAMU poultry facility for the study, and samples were collected over a five-day period. Bioaerosol sampling was conducted using a Wetted Wall Cyclone (WWC) bioaerosol collector at the two CAFU houses, in which House A housed approximately 720 broiler chickens and roosters, while House B remained unoccupied and served as a reference. Both houses consisted of 24 pens arranged on either side of a central walkway. Bacterial content analysis was conducted using microbial plating, real-time Polymerase Chain Reaction (PCR), and Fatty Acid Methyl Ester (FAME) analysis, while ambient temperature and relative humidity were also monitored. The concentrations of microorganisms in House A showed a highly dynamic range, ranging from 4000 to 60,000 colony forming units (CFU) per cubic meter of air. Second, the WWC samples contained approximately ten-fold more bacterial DNA than the filter samples, suggesting higher levels of viable cells captured by the WWC. Third, significant concentrations of pathogens, including , , and , were detected in the poultry facility. Lastly, the WWC system demonstrated effective functionality and continuous operation, even in the challenging sampling environment of the CAFU. The goal of this study was to characterize the resident population of microorganisms (pathogenic and non-pathogenic) present in the CAFUs and to evaluate the WWC's performance in such an environment characterized by elevated temperature, high dust content, and feathers. This knowledge could then be used to improve understanding microorganism dynamics in CAFUs including the spread of bacterial infections between animals and from animals to humans that work in these facilities, as well as of the WWC performance in this type of environment (elevated temperature, high content of dust and feathers). A more comprehensive understanding can aid in improving the management of bacterial infections in these settings.
从德克萨斯农工大学家禽养殖设施中选取了两个家禽密闭式动物饲养单元（CAFUs），即“A舍”和“B舍”进行研究，并在五天时间内收集样本。在这两个CAFU鸡舍中使用湿壁旋风器（WWC）生物气溶胶收集器进行生物气溶胶采样，其中A舍饲养了约720只肉鸡和公鸡，而B舍为空舍，用作对照。两舍均由位于中央通道两侧的24个围栏组成。使用微生物平板培养、实时聚合酶链反应（PCR）和脂肪酸甲酯（FAME）分析进行细菌含量分析，同时还监测环境温度和相对湿度。A舍中的微生物浓度显示出高度动态范围，每立方米空气中的菌落形成单位（CFU）从4000到60000不等。其次，WWC样本中的细菌DNA比过滤器样本多约十倍，这表明WWC捕获的活细胞水平更高。第三，在家禽养殖设施中检测到了包括[此处原文缺失具体病原体名称]等病原体的显著浓度。最后，即使在CAFU具有挑战性的采样环境中，WWC系统也展示了有效的功能和持续运行能力。本研究的目的是表征CAFUs中存在的微生物（致病和非致病）种群，并评估WWC在这种以高温、高粉尘含量和羽毛为特征的环境中的性能。这些知识随后可用于增进对CAFUs中微生物动态的理解，包括动物之间以及从动物到在这些设施中工作的人类的细菌感染传播，以及WWC在这种环境类型（高温、高粉尘和羽毛含量）中的性能。更全面的理解有助于改善这些环境中细菌感染的管理。
