Aix Marseille Univ, Inserm, INMED, Turing Center for Living Systems, Marseille, France.
Wavefront-Engineering Microscopy Group, Photonics Department, Vision Institute, Sorbonne University, INSERM, CNRS, Paris, France.
Nat Neurosci. 2023 Sep;26(9):1555-1565. doi: 10.1038/s41593-023-01405-5. Epub 2023 Aug 31.
Spontaneous synchronous activity is a hallmark of developing brain circuits and promotes their formation. Ex vivo, synchronous activity was shown to be orchestrated by a sparse population of highly connected GABAergic 'hub' neurons. The recent development of all-optical methods to record and manipulate neuronal activity in vivo now offers the unprecedented opportunity to probe the existence and function of hub cells in vivo. Using calcium imaging, connectivity analysis and holographic optical stimulation, we show that single GABAergic, but not glutamatergic, neurons influence population dynamics in the barrel cortex of non-anaesthetized mouse pups. Single GABAergic cells mainly exert an inhibitory influence on both spontaneous and sensory-evoked population bursts. Their network influence scales with their functional connectivity, with highly connected hub neurons displaying the strongest impact. We propose that hub neurons function in tailoring intrinsic cortical dynamics to external sensory inputs.
自发性同步活动是发育中大脑回路的标志，并促进其形成。在体外，同步活动被证明是由一小部分高度连接的 GABA 能“中枢”神经元协调的。最近开发的用于在体内记录和操纵神经元活动的全光学方法，现在为在体内探测中枢细胞的存在和功能提供了前所未有的机会。使用钙成像、连接分析和全息光刺激，我们表明，在非麻醉的幼鼠桶状皮层中，只有 GABA 能神经元，而不是谷氨酸能神经元，会影响群体动力学。单个 GABA 能神经元主要对自发性和感觉诱发的群体爆发产生抑制影响。它们的网络影响与功能连接性成正比，具有高度连接性的中枢神经元具有最强的影响。我们提出中枢神经元的功能是将皮质固有动力学调整为外部感觉输入。
