Wang Jing, Sheng Zhicun, Liu Yunying, Chen Xiaolan, Wang Shuaibing, Yang Haifeng
Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, Jiangsu Province, China.
Zhongchong Sino Biotech Taizhou Co., Ltd., Taizhou, Jiangsu Province, China.
Front Pharmacol. 2023 Aug 16;14:1178177. doi: 10.3389/fphar.2023.1178177. eCollection 2023.
is a zoonotic opportunistic pathogen that represents a significant threat to public health. Previous studies have shown that tannic acid (TA) has an inhibitory effect on a variety of bacteria. In this study, the proteome and transcriptome of were analyzed to comprehensively assess changes in genes and proteins induced by TA. Initial observations of morphological changes revealed that TA damaged the integrity of the cell membrane. Next, proteomic and genetic analyses showed that exposure to TA altered the expression levels of 651 differentially expressed proteins (DEPs, 283 upregulated and 368 downregulated) and 503 differentially expressed genes (DEGs, 191 upregulated and 312 downregulated). Analysis of the identified DEPs and DEGs suggested that TA damages the integrity of the cell envelope by decreasing the expression and protein abundance of enzymes involved in the synthesis of peptidoglycans, teichoic acids and fatty acids, such as , and . After treatment with TA, the assembly of ribosomes in was severely impaired by significant reductions in available ribosome components, and thus protein synthesis was hindered. The levels of genes and proteins associated with amino acids and purine synthesis were remarkably decreased, which further reduced bacterial viability. In addition, ABC transporters, which are involved in amino acid and ion transport, were also badly affected. Our results reveal the molecular mechanisms underlying the effects of TA on and provide a theoretical basis for the application of TA as an antibacterial chemotherapeutic agent.
是一种人畜共患的机会性病原体，对公众健康构成重大威胁。先前的研究表明，单宁酸（TA）对多种细菌具有抑制作用。在本研究中，对[具体物种名称缺失]的蛋白质组和转录组进行了分析，以全面评估TA诱导的基因和蛋白质变化。对形态变化的初步观察表明，TA破坏了细胞膜的完整性。接下来，蛋白质组学和基因分析表明，暴露于TA会改变651种差异表达蛋白（DEPs，283种上调和368种下调）和503种差异表达基因（DEGs，191种上调和312种下调）的表达水平。对鉴定出的DEPs和DEGs的分析表明，TA通过降低参与肽聚糖、磷壁酸和脂肪酸合成的酶（如[具体酶名称缺失]、[具体酶名称缺失]和[具体酶名称缺失]）的表达和蛋白质丰度来破坏细胞壁的完整性。用TA处理后，[具体物种名称缺失]中核糖体的组装因可用核糖体成分的显著减少而严重受损，从而阻碍了蛋白质合成。与氨基酸和嘌呤合成相关的基因和蛋白质水平显著降低，这进一步降低了细菌的活力。此外，参与氨基酸和离子运输的ABC转运蛋白也受到严重影响。我们的结果揭示了TA对[具体物种名称缺失]作用的分子机制，并为TA作为抗菌化疗药物的应用提供了理论依据。
