Amani Mahdiyar, Rakhshani Amir, Maghsoudian Samane, Rasoulzadehzali Monireh, Yoosefi Sepideh, Keihankhadiv Shadi, Fatahi Yousef, Darbasizadeh Behzad, Ebrahimi Seyed-Mostafa, Ejarestaghi Negin Mousavi, Farhadnejad Hassan, Motasadizadeh Hamidreza
Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences, Tehran, Iran; Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2023 Sep 30;249:126705. doi: 10.1016/j.ijbiomac.2023.126705. Epub 2023 Sep 9.
A pH-sensitive bilayer electrospun nanofibrous mat containing both antibiotic (gentamicin sulfate, GEN) and non-steroidal anti-inflammatory (diclofenac sodium, DIC) drugs was fabricated for burn wound dressing by electrospinning technique, in which ethyl cellulose (EC) and ethyl cellulose/Eudragit S-100 (EC/ES-100) formed the top and bottom layers, respectively. The fabricated pH-sensitive bilayer electrospun nanofibrous mats were characterized from aspects of both structure and efficiency. Physicochemical properties were investigated via SEM, FTIR, and TGA. The swelling ratio and in vitro drug release of the fabricated nanofibrous mats were studied in different pHs. MTT was applied to assess the safety of the fiber mats. Finally, the in vivo efficiency of the designed pH-sensitive bilayer electrospun nanofibrous mats was examined on the male Wistar rats. Based on the histological analysis and wound healing test (in vivo animal experiments), the (ES100/EC-DIC/GEN)-(EC) pH-sensitive bilayer nanofibrous mat displayed faster wound healing than other bilayer nanofibrous mat. As a result, (ES100/EC-DIC/GEN)-(EC) bilayer nanofibrous mat with pH-responsion could accelerate the burn wound healing process via decreasing the adverse effects of GEN and DIC as topical antimicrobial and anti-inflammatory agents, receptively.
通过静电纺丝技术制备了一种含有抗生素（硫酸庆大霉素，GEN）和非甾体抗炎药（双氯芬酸钠，DIC）的pH敏感双层静电纺纳米纤维垫用于烧伤创面敷料，其中乙基纤维素（EC）和乙基纤维素/ Eudragit S - 100（EC/ES - 100）分别形成顶层和底层。从结构和效率方面对制备的pH敏感双层静电纺纳米纤维垫进行了表征。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和热重分析（TGA）研究了其物理化学性质。研究了制备的纳米纤维垫在不同pH值下的溶胀率和体外药物释放情况。采用MTT法评估纤维垫的安全性。最后，在雄性Wistar大鼠上考察了所设计的pH敏感双层静电纺纳米纤维垫的体内效果。基于组织学分析和伤口愈合试验（体内动物实验），（ES100/EC - DIC/GEN）-（EC）pH敏感双层纳米纤维垫显示出比其他双层纳米纤维垫更快的伤口愈合速度。因此，具有pH响应性的（ES100/EC - DIC/GEN）-（EC）双层纳米纤维垫可分别通过降低GEN和DIC作为局部抗菌和抗炎剂的不良反应来加速烧伤创面愈合过程。
