Molecular and Experimental Mycobacteriology Group, Research Center Borstel, Leibniz Lung Center, Borstel, Germany.
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Front Public Health. 2023 Aug 3;11:1204064. doi: 10.3389/fpubh.2023.1204064. eCollection 2023.
Targeted next-generation sequencing (tNGS) from clinical specimens has the potential to become a comprehensive tool for routine drug-resistance (DR) prediction of complex strains (MTBC), the causative agent of tuberculosis (TB). However, TB mainly affects low- and middle-income countries, in which the implementation of new technologies have specific needs and challenges. We propose a model for programmatic implementation of tNGS in settings with no or low previous sequencing capacity/experience. We highlight the major challenges and considerations for a successful implementation. This model has been applied to build NGS capacity in Namibia, an upper middle-income country located in Southern Africa and suffering from a high-burden of TB and TB-HIV, and we describe herein the outcomes of this process.
靶向下一代测序（tNGS）可从临床标本中获得，有望成为一种全面的工具，用于常规预测耐药性（DR）复杂菌株（MTBC），MTBC 是结核病（TB）的病原体。然而，TB 主要影响低收入和中等收入国家，这些国家在实施新技术方面有特定的需求和挑战。我们提出了一种在以前没有或很少有测序能力/经验的环境中进行靶向测序的规划实施模型。我们强调了成功实施的主要挑战和考虑因素。该模型已应用于在纳米比亚建立 NGS 能力，纳米比亚是位于南部非洲的一个中上收入国家，结核病和 TB-HIV 负担沉重，本文描述了这一过程的结果。
