The Detection of Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers.

Wheat stripe rust is a fungal disease caused by f. sp. (). It significantly impacts wheat yields in Xinjiang, China. Breeding and promoting disease-resistant cultivars carrying disease-resistance genes remains the most cost-effective strategy with which to control the disease. In this study, 17 molecular markers were used to identify , , , , , , , , , and in 82 wheat cultivars from Xinjiang. According to the differences in SNP loci, the KASP markers for , , , , and were designed and detected in the same set of 82 wheat cultivars. The results showed that there was a diverse distribution of genes across all wheat cultivars in Xinjiang, and the detection rates of , , , , , and were the highest, ranging from 74.39% to 98.78%. In addition, and were prevalent in spring wheat cultivars, with detection rates of 100% and 97.56%, respectively. A substantial 85.37% of wheat cultivars carried at least six or more different combinations of genes. The cultivar Xindong No.15 exhibited the remarkable presence of 11 targeted genes. The pedigree analysis results showed that 33.33% of Xinjiang wheat cultivars shared similar parentage, potentially leading to a loss of resistance against . The results clarified the gene distribution of the Xinjiang wheat cultivars and screened out varieties with a high resistance against .