Jovanović Aleksandra A, Balanč Bojana, Volić Mina, Pećinar Ilinka, Živković Jelena, Šavikin Katarina P
Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia.
Innovation Centre of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Plants (Basel). 2023 Aug 25;12(17):3063. doi: 10.3390/plants12173063.
In the present study, rosehip ( L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was -22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome-extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.
在本研究中，采用一种名为前体脂质体法的单步程序成功地将玫瑰果提取物包封在磷脂脂质体中。将部分所得脂质体进行紫外线照射，对未处理（天然）和紫外线照射的脂质体在包封效率、化学成分（高效液相色谱分析）、抗氧化能力、粒径、多分散指数、zeta电位、电导率、迁移率和抗氧化能力方面进行进一步表征。应用拉曼光谱以及差示扫描量热法来评估紫外线照射对脂质体物理化学性质的影响。负载提取物的脂质体的包封效率高于90%；平均粒径为251.5 nm；zeta电位为-22.4 mV；电导率为0.007 mS/cm。紫外线照射并未使上述参数发生变化。此外，照射并未影响脂质体-提取物体系的抗氧化潜力。拉曼光谱表明，在脂质体包封过程中提取物被脂质膜完全覆盖，并且脂质体中玫瑰果提取物的存在使过氧化过程降至最低。这些结果可能会指导负载玫瑰果提取物的脂质体在食品、制药或化妆品行业的潜在应用，特别是在需要脂质体灭菌时。
