Zhao Xiaotong, Goher Farhan, Chen Lei, Song Jiancheng, Zhao Jiqiang
School of Life Science, Yantai University, Yantai 264005, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China.
Plants (Basel). 2023 Aug 25;12(17):3065. doi: 10.3390/plants12173065.
The subtilisin-like protease (SBT) family is widely known for its role in stress resistance to a number of stressors in different plant species, but is rarely studied in wheat. Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that hydrolyze proteins into small peptides, which bind to receptors as signal molecules or ligands and participate in signal transduction. In this study, we identified 255 putative SBT genes from the wheat reference genome and then divided these into seven clades. Subsequently, we performed syntenic relation analysis, exon-intron organization, motif composition, and -element analysis. Further, expression analysis based on RNA-seq and tissue-specific expression patterns revealed that gene family expression has multiple intrinsic functions during various abiotic and biotic stresses. Analysis of RNA-seq expression assays and further validation through qRT PCR suggested that some of the genes have significant changes in expression levels during interaction. , , , and genes showed increasing expression levels during compatible and non-compatible interactions, while the expression levels of and showed a decreasing trend, indicating that these members of the wheat gene family may have a role in wheat's defense against pathogens. In conclusion, these results expand our understanding of the gene family, and provide a valuable reference for future research on the stress resistance function and comprehensive data of wheat SBT members.
枯草杆菌蛋白酶样蛋白酶（SBT）家族因其在不同植物物种中对多种应激源的抗逆作用而广为人知，但在小麦中却鲜有研究。枯草杆菌蛋白酶样丝氨酸蛋白酶（SBTs）是一种丝氨酸蛋白水解酶，可将蛋白质水解成小肽，这些小肽作为信号分子或配体与受体结合并参与信号转导。在本研究中，我们从小麦参考基因组中鉴定出255个假定的SBT基因，然后将它们分为七个进化枝。随后，我们进行了共线性关系分析、外显子-内含子组织分析、基序组成分析和元件分析。此外，基于RNA测序的表达分析和组织特异性表达模式表明，该基因家族在各种非生物和生物胁迫过程中具有多种内在功能。RNA测序表达分析及通过qRT-PCR进一步验证表明，部分基因在相互作用过程中表达水平发生显著变化。TaSBT2D、TaSBT3A、TaSBT3B和TaSBT5A基因在亲和与非亲和相互作用过程中表达水平升高，而TaSBT3D和TaSBT5B的表达水平呈下降趋势，表明小麦SBT基因家族的这些成员可能在小麦抵御病原体中发挥作用。总之，这些结果扩展了我们对SBT基因家族的理解，并为未来小麦SBT成员抗逆功能及综合数据的研究提供了有价值的参考。
