Liotti F S, Bodo M, Menghini A R, Guerrieri P, Pezzetti F
Cancer Lett. 1986 Oct;33(1):99-106. doi: 10.1016/0304-3835(86)90106-0.
The authors studied the effects of a treatment with ascorbic acid on in vitro multiplication of ascites tumour cells (ATP C+), of fibroblast-like cells and of hepatocytes from chick embryos, by measuring [3H]thymidine incorporation into DNA. The results obtained show that the ATP C+ cells are the most sensitive to the toxic effects of the experimental treatment, while the hepatocytes are the most resistant cell population. A treatment with catalase was able to greatly reduce the damage caused by ascorbic acid on the ATP C+ cells. It is hypothesized that ascorbic acid inhibits cell multiplication by the H2O2 formed by its oxidation and that the cells having the highest level of catalase are more resistant to its toxic effects.
作者通过测量[3H]胸腺嘧啶核苷掺入DNA的情况，研究了抗坏血酸处理对腹水肿瘤细胞（ATP C+）、成纤维细胞样细胞和鸡胚肝细胞体外增殖的影响。所得结果表明，ATP C+细胞对实验处理的毒性作用最为敏感，而肝细胞是最具抗性的细胞群体。过氧化氢酶处理能够大大降低抗坏血酸对ATP C+细胞造成的损伤。据推测，抗坏血酸通过其氧化形成的H2O2抑制细胞增殖，而过氧化氢酶水平最高的细胞对其毒性作用更具抗性。
