Department of Biotechnology, Lakehead University, Thunder Bay, ON, Canada.
Biorefining Research Institute (BRI), Lakehead University, Thunder Bay, ON, Canada.
Biodegradation. 2024 Jul;35(4):341-360. doi: 10.1007/s10532-023-10048-z. Epub 2023 Sep 9.
Enzymatic degradation of polyethylene terephthalic acid (PET) has been gaining increasing importance. This has resulted in a significant increase in the search for newer enzymes and the development of more efficient enzyme-based systems. Due to the lack of a standard screening process, screening new enzymes has relied on other assays to determine the presence of esterase activity. This, in turn, has led to various nomenclatures and methods used to describe them and measure their activity. Since all PET-hydrolyzing enzymes are α/β hydrolases, they catalyze a serine nucleophilic attack and cleave an ester bond. They are lipases, esterases, cutinases and hydrolases. This has been used interchangeably, leading to difficulties while comparing results and evaluating progress. This review discusses the varied enzyme nomenclature being adapted, the different assays and analysis methods reported, and the strategies used to increase PET-hydrolyzing enzyme efficiency. A section on the various ways to quantify PET hydrolysis is also covered.
聚对苯二甲酸乙二醇酯（PET）的酶促降解越来越受到重视。这导致人们越来越多地寻找新的酶，并开发更有效的基于酶的系统。由于缺乏标准的筛选过程，筛选新酶依赖于其他测定法来确定酯酶活性的存在。这反过来又导致了各种命名法和方法被用来描述它们并测量它们的活性。由于所有 PET 水解酶都是 α/β 水解酶，它们催化丝氨酸亲核攻击并裂解酯键。它们是脂肪酶、酯酶、角质酶和水解酶。这些术语可以互换使用，这导致在比较结果和评估进展时出现困难。这篇综述讨论了正在采用的各种酶命名法、报道的不同测定法和分析方法，以及用于提高 PET 水解酶效率的策略。还涵盖了定量 PET 水解的各种方法。
