Guangxi Key Laboratory of Agro-Environment and Agro-Product Safety, National Demonstration Center for Experimental Plant Science Education, Agricultural College, Guangxi University, 100 University Road, Nanning, Guangxi, 530004, P.R. China.
Guangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, 530007, Guangxi, P.R. China.
BMC Plant Biol. 2023 Sep 14;23(1):427. doi: 10.1186/s12870-023-04446-x.
To elucidate the mechanisms underlying the resistance to smut of different sugarcane cultivars, endophytic bacterial and fungal compositions, functions and metabolites in the stems of the sugarcane cultivars were analyzed using high-throughput sequencing techniques and nontargeted metabolomics. The results showed that the levels of ethylene, salicylic acid and jasmonic acid in sugarcane varieties that were not sensitive to smut were all higher than those in sensitive sugarcane varieties. Moreover, endophytic fungi, such as Ramichloridium, Alternaria, Sarocladium, Epicoccum, and Exophiala species, could be considered antagonistic to sugarcane smut. Additionally, the highly active arginine and proline metabolism, pentose phosphate pathway, phenylpropanoid biosynthesis, and tyrosine metabolism in sugarcane varieties that were not sensitive to smut indicated that these pathways contribute to resistance to smut. All of the above results suggested that the relatively highly abundant antagonistic microbes and highly active metabolic functions of endophytes in non-smut-sensitive sugarcane cultivars were important for their relatively high resistance to smut.
为了阐明不同甘蔗品种对黑穗病抗性的机制，本研究采用高通量测序技术和非靶向代谢组学方法分析了甘蔗品种茎内的内生细菌和真菌组成、功能和代谢物。结果表明，对黑穗病不敏感的甘蔗品种的乙烯、水杨酸和茉莉酸水平均高于敏感甘蔗品种。此外，内生真菌如拟盘多毛孢属、链格孢属、层出镰刀菌属、匐柄霉属和外瓶霉属等可被视为甘蔗黑穗病的拮抗真菌。此外，对黑穗病不敏感的甘蔗品种中具有较高活性的精氨酸和脯氨酸代谢、戊糖磷酸途径、苯丙素生物合成和酪氨酸代谢途径表明，这些途径有助于抵抗黑穗病。上述结果表明，非黑穗病敏感甘蔗品种中相对丰富的拮抗微生物和内生菌的高活性代谢功能对其较高的黑穗病抗性至关重要。
