Research Center for Advanced Materials, Faculty of Materials Engineering, Sahand University of Technology, P.O. Box: 51335-1996, Tabriz, Iran.
Molecular Medicine Department (DMM), Center for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli 3/B, 27100, Pavia, Italy.
Sci Rep. 2023 Sep 25;13(1):16045. doi: 10.1038/s41598-023-43393-3.
NiTi is a class of metallic biomaterials, benefit from superelastic behavior, high biocompatibility, and favorable mechanical properties close to that of bone. However, the Ni ion leaching, poor bioactivity, and antibacterial activity limit its clinical applications. In this study, HAp-NbO composite layers were PC electrodeposited from aqueous electrolytes containing different concentrations of the NbO particles, i.e., 0-1 g/L, to evaluate the influence of the applied surface engineering strategy on in vitro immersion behavior, Ni ion leaching level, and antibacterial activity of the bare NiTi. Surface characteristics of the electrodeposited layers were analyzed using SEM, TEM, XPS, and AFM. The immersion behavior of the samples was comprehensively investigated through SBF and long-term PBS soaking. Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) infective reference bacteria were employed to address the antibacterial activity of the samples. The results illustrated that the included particles led to more compact and smoother layers. Unlike bare NiTi, composite layers stimulated apatite formation upon immersion in both SBF and PBS media. The concentration of the released Ni ion from the composite layer, containing 0.50 g/L NbO was ≈ 60% less than that of bare NiTi within 30 days of immersion in the corrosive PBS solution. The NbO-reinforced layers exhibited high anti-adhesive activity against both types of pathogenic bacteria. The hybrid metallic-ceramic system comprising HAp-NbO-coated NiTi offers the prospect of a potential solution for clinical challenges facing the orthopedic application of NiTi.
镍钛是一类金属生物材料,得益于超弹性、高生物相容性和与骨骼相近的理想机械性能。然而,镍离子浸出、较差的生物活性和抗菌活性限制了其临床应用。在这项研究中,通过在含有不同浓度 NbO 颗粒(即 0-1 g/L)的水溶液中进行 PC 电沉积,在裸镍钛上制备 HAp-NbO 复合层,以评估应用表面工程策略对体外浸泡行为、镍离子浸出水平和抗菌活性的影响。采用 SEM、TEM、XPS 和 AFM 分析了电沉积层的表面特性。通过 SBF 和长期 PBS 浸泡综合研究了样品的浸泡行为。采用革兰氏阴性大肠杆菌(E. coli)和革兰氏阳性金黄色葡萄球菌(S. aureus)感染参考细菌评估了样品的抗菌活性。结果表明,包含的颗粒导致了更致密和更光滑的层。与裸镍钛不同,复合层在 SBF 和 PBS 介质中浸泡时均能刺激磷灰石的形成。在腐蚀性 PBS 溶液中浸泡 30 天内,含有 0.50 g/L NbO 的复合层释放的镍离子浓度比裸镍钛低约 60%。增强 NbO 的层对两种类型的致病菌均表现出高抗粘附活性。由 HAp-NbO 涂层镍钛组成的混合金属-陶瓷系统为解决镍钛在骨科应用中面临的临床挑战提供了潜在的解决方案。