Jung Hyun Jung, Lee Won
Department of Dentistry, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Republic of Korea.
Heliyon. 2023 Sep 20;9(9):e20303. doi: 10.1016/j.heliyon.2023.e20303. eCollection 2023 Sep.
The objective of this study is to compare the microbiome of healthy peri-implant sulcus (C) and peri-implantitis sulcus (U) from the same patient and analyze the difference in the microbiome composition.
DNA samples of subgingival biofilms from 10 C (control group) and 10 U (uncontrolled group) sites were sent to Microbiome Center in Korea Research Institute of Biomedical Science and analyzed using 16s rRNA gene amplification and sequencing (MiSeq, Illumina) and human oral microbiome database (HOMD).
At the phylum level, and were more abundant in group C, while and were dominant in group U. At the genus level, the core peri-implant microbiome was in group C. On the other hand, the core peri-implant microbiome was especially in group U.
In this study, the microbiome composition of peri-implantitis sulcus was different from that of healthy peri-implant sulcus from the same patient. The peri-implantitis microbiome was pathogen-enriched and was similar to the microbiome associated with periodontitis.
本研究的目的是比较同一患者健康种植体周围龈沟（C组）和种植体周炎龈沟（U组）的微生物群，并分析微生物群组成的差异。
将来自10个C组（对照组）和10个U组（未控制组）部位的龈下生物膜DNA样本送至韩国生物医学科学研究所微生物组中心，采用16s rRNA基因扩增和测序（MiSeq，Illumina）以及人类口腔微生物组数据库（HOMD）进行分析。
在门水平上，C组中[具体菌门1]和[具体菌门2]更为丰富，而U组中[具体菌门3]和[具体菌门4]占主导地位。在属水平上，C组中种植体周围核心微生物群是[具体菌属1]。另一方面，U组中种植体周围核心微生物群尤其是[具体菌属2]。
在本研究中，同一患者种植体周炎龈沟的微生物群组成与健康种植体周围龈沟不同。种植体周炎微生物群富含病原体，与牙周炎相关的微生物群相似。
