Sahrmann Philipp, Gilli Fabienne, Wiedemeier Daniel B, Attin Thomas, Schmidlin Patrick R, Karygianni Lamprini
Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, CH-8032 Zurich, Switzerland.
Statistical Services, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, CH-8032 Zurich, Switzerland.
Microorganisms. 2020 May 1;8(5):661. doi: 10.3390/microorganisms8050661.
This review aimed to systematically compare microbial profiles of peri-implantitis to those of periodontitis and healthy implants. Therefore, an electronic search in five databases was conducted. For inclusion, studies assessing the microbiome of peri-implantitis in otherwise healthy patients were considered. Literature was assessed for consistent evidence of exclusive or predominant peri-implantitis microbiota. Of 158 potentially eligible articles, data of 64 studies on 3730 samples from peri-implant sites were included in this study. Different assessment methods were described in the studies, namely bacterial culture, PCR-based assessment, hybridization techniques, pyrosequencing, and transcriptomic analyses. After analysis of 13 selected culture-dependent studies, no microbial species were found to be specific for peri-implantitis. After assessment of 28 studies using PCR-based methods and a meta-analysis on 19 studies, a higher prevalence of and (log-odds ratio 4.04 and 2.28, respectively) was detected in peri-implantitis biofilms compared with healthy implants. spp., spp. and spp. were found in all five pyrosequencing studies in healthy-, periodontitis-, and peri-implantitis samples. In conclusion, the body of evidence does not show a consistent specific profile. Future studies should focus on the assessment of sites with different diagnosis for the same patient, and investigate the complex host-biofilm interaction.
本综述旨在系统比较种植体周围炎与牙周炎及健康种植体的微生物谱。因此，在五个数据库中进行了电子检索。纳入标准为评估健康患者种植体周围炎微生物群的研究。对文献进行评估，以寻找种植体周围炎微生物群具有排他性或优势性的一致证据。在158篇可能符合条件的文章中，本研究纳入了64项研究的数据，这些研究涉及来自种植体周围部位的3730个样本。研究中描述了不同的评估方法，即细菌培养、基于PCR的评估、杂交技术、焦磷酸测序和转录组分析。在对13项选定的依赖培养的研究进行分析后，未发现有微生物物种对种植体周围炎具有特异性。在对28项使用基于PCR方法的研究进行评估以及对19项研究进行荟萃分析后，发现与健康种植体相比，种植体周围炎生物膜中 和 的患病率更高（对数优势比分别为4.04和2.28）。在所有五项关于健康、牙周炎和种植体周围炎样本的焦磷酸测序研究中均发现了 属、 属和 属。总之，现有证据并未显示出一致的特定谱型。未来的研究应侧重于对同一患者不同诊断部位的评估，并研究复杂的宿主-生物膜相互作用。
