Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Pediatr Res. 2024 Feb;95(3):641-646. doi: 10.1038/s41390-023-02838-1. Epub 2023 Oct 13.
Osteopontin (OPN) is an important breastmilk protein involved in infant intestinal, immunological, and brain development. However, little is known about how common milk pasteurization and storage techniques affect this important bioactive protein.
Human milk osteopontin concentration was measured in single-donor fresh (n = 1) or frozen (n = 20) breastmilk, pooled Holder-pasteurized donor breastmilk (n = 11), and a shelf-stable (retort pasteurized) breastmilk product (n = 2) by ELISA. Single-donor breastmilk samples were subjected to pasteurization and/or freezing before measuring osteopontin concentrations.
Holder pasteurization of breastmilk resulted in an ∼50% decrease in osteopontin concentration within single-donor samples. Breastmilk from mothers of preterm infants trended toward higher osteopontin concentration than mothers of term infants; however, samples from preterm mothers experienced greater osteopontin degradation upon pasteurization. A commercial breastmilk product that underwent retort pasteurization had lower osteopontin concentration than a Holder-pasteurized pooled breastmilk product. Finally, freezing breastmilk prior to Holder pasteurization resulted in less osteopontin degradation than Holder pasteurization prior to freezing.
Commonly used breastmilk pasteurization and storage techniques, including freezing and Holder pasteurization, decrease the concentration of the bioactive protein osteopontin in human breastmilk. Holder pasteurization reduced osteopontin concentration by an average of 63%, while freezing resulted in an 8-12% decrease.
Pasteurization of human breastmilk significantly decreases the concentration of the bioactive protein osteopontin. Use of both pasteurization and freezing techniques for breastmilk preservation results in greater loss of osteopontin. This study presents for the first time an analysis of osteopontin concentrations in single-donor pasteurized milk samples.
骨桥蛋白（OPN）是一种重要的母乳蛋白，参与婴儿的肠道、免疫和大脑发育。然而，对于常见的牛奶巴氏消毒和储存技术如何影响这种重要的生物活性蛋白，人们知之甚少。
通过 ELISA 法测量了单份新鲜（n=1）或冷冻（n=20）母乳、 pooled Holder 巴氏消毒供体母乳（n=11）和货架稳定（高压灭菌）母乳产品（n=2）中的人乳骨桥蛋白浓度。在测量骨桥蛋白浓度之前，对单份母乳样本进行巴氏消毒和/或冷冻。
Holder 巴氏消毒母乳会导致单份样本中的骨桥蛋白浓度降低约 50%。早产儿母亲的母乳骨桥蛋白浓度趋于高于足月儿母亲，但早产儿母亲的样本在巴氏消毒后骨桥蛋白降解更多。经过高压灭菌的商业母乳产品的骨桥蛋白浓度低于经过 Holder 巴氏消毒的 pooled 母乳产品。最后，Holder 巴氏消毒前冷冻母乳比 Holder 巴氏消毒前冷冻导致的骨桥蛋白降解更少。
常用的母乳巴氏消毒和储存技术，包括冷冻和 Holder 巴氏消毒，会降低人乳中生物活性蛋白骨桥蛋白的浓度。Holder 巴氏消毒平均降低骨桥蛋白浓度 63%，而冷冻导致骨桥蛋白浓度降低 8-12%。
巴氏消毒人乳会显著降低生物活性蛋白骨桥蛋白的浓度。巴氏消毒和冷冻技术的联合使用会导致骨桥蛋白的大量损失。本研究首次对单份巴氏消毒牛奶样本中的骨桥蛋白浓度进行了分析。
