Escolà Glòria, González-Miguel Víctor M, Campo Sonia, Catala-Forner Mar, Domingo Concha, Marqués Luis, San Segundo Blanca
Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), C/de la Vall Moronta, CRAG Building, 08193 Barcelona, Spain.
Institute of Agrifood Research and Technology (IRTA), Field Crops, Ctra. Balada km. 1, 43870 Tarragona, Spain.
Plants (Basel). 2023 Oct 11;12(20):3536. doi: 10.3390/plants12203536.
Rice is one of the most important crops in the world, and its production is severely affected by the rice blast disease caused by the fungus . Several major blast resistance genes and QTLs associated with blast resistance have been described and mostly identified in rice varieties. In this work, we report the obtention of a blast-resistant rice breeding line derived from crosses between the resistant variety CT13432 and the elite cultivar JSendra (highly susceptible to blast). The breeding line, named COPSEMAR9, was found to exhibit resistance to leaf blast and panicle blast, as demonstrated by disease assays under controlled and field conditions. Furthermore, a high-quality genome sequence of the blast-resistant breeding line was obtained using a strategy that combines short-read sequencing (Illumina sequencing) and long-read sequencing (Pacbio sequencing). The use of a whole-genome approach allowed the fine mapping of DNA regions of and origin present in the COPSEMAR9 genome and the identification of parental gene regions potentially contributing to blast resistance in the breeding line. Rice blast resistance genes (including derived from the resistant parent) and defense-related genes in the genome of COPSEMAR9 were identified. Whole-genome analyses also revealed the presence of microRNAs (miRNAs) with a known function in the rice response to infection in COPSEMAR9, which might also contribute to its phenotype of blast resistance. From this study, the genomic information and analysis methods provide valuable knowledge that will be useful in breeding programs for blast resistance in rice cultivars.
水稻是世界上最重要的作物之一，其产量受到由真菌引起的稻瘟病的严重影响。已经描述了几个与抗稻瘟病相关的主要抗瘟基因和数量性状位点（QTL），并且大多在水稻品种中得到鉴定。在这项工作中，我们报告了从抗性品种CT13432与优良品种JSendra（对稻瘟病高度敏感）杂交获得的一个抗稻瘟病水稻育种系。通过在可控条件和田间条件下的病害测定表明，这个名为COPSEMAR9的育种系对叶瘟和穗瘟表现出抗性。此外，使用一种结合短读长测序（Illumina测序）和长读长测序（Pacbio测序）的策略，获得了该抗稻瘟病育种系的高质量基因组序列。采用全基因组方法能够精细定位COPSEMAR9基因组中存在的来自 和 来源的DNA区域，并鉴定出可能对该育种系的抗瘟性有贡献的亲本基因区域。鉴定了COPSEMAR9基因组中的水稻抗稻瘟病基因（包括来自抗性亲本的 ）和防御相关基因。全基因组分析还揭示了COPSEMAR9中在水稻对 感染反应中具有已知功能的微小RNA（miRNA）的存在，这也可能有助于其抗稻瘟病表型。从这项研究中，基因组信息和分析方法提供了有价值的知识，这将有助于水稻品种抗稻瘟病的育种计划。
