Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran.
Sci Rep. 2023 Oct 31;13(1):18704. doi: 10.1038/s41598-023-45921-7.
New topical antibacterial agents are required to inhibit and development of bacteria and also promoting the wound healing process. This study was evaluating the healing effect of Myristica fragrans extract coated with carboxymethyl cellulose, zinc oxide and graphite carbon nitride (CMC/ZnO/g-C3N4/MyR) by photocatalytic process on the healing process of full-thickness infectious excision wounds in mice. Nanosheets were prepared and physicochemical properties were evaluated. Safety, in vitro release, antibacterial activities under in vitro and in vivo condition, wound contraction, histopathological properties and the protein expressions of tumor necrosis factor-α (TNF-α), collagen 1A (COL1A) and CD31 were also evaluated. Physicochemical properties confirmed their successful synthesis. Nanosheets exhibited antibacterial activity under in vitro and in vivo conditions. The formulations containing CMC/ZnO/g-C3N4/MyR, significantly (P < 0.05) competed with standard ointment of mupirocin for accelerating the wound healing process due to their effects on bacterial count and the expression of TNF-α and also accelerating the proliferative phase. This structure can be used as a safe structure in combination with other agents for accelerating the wound healing process following future clinical studies.
需要新的局部抗菌剂来抑制和阻止细菌的发展，同时促进伤口愈合过程。本研究通过光催化过程评估涂有羧甲基纤维素、氧化锌和石墨氮化碳（CMC/ZnO/g-C3N4/MyR）的肉豆蔻提取物对小鼠全层感染性切除伤口愈合过程的愈合效果。制备纳米片并评估其物理化学性质。评估安全性、体外释放、体外和体内条件下的抗菌活性、伤口收缩、组织病理学特性以及肿瘤坏死因子-α (TNF-α)、胶原蛋白 1A (COL1A) 和 CD31 的蛋白表达。物理化学性质证实了它们的成功合成。纳米片在体外和体内条件下均表现出抗菌活性。由于 CMC/ZnO/g-C3N4/MyR 制剂对细菌计数和 TNF-α 的表达有影响，并且加速了增殖期，因此与莫匹罗星标准软膏相比，显著（P<0.05）加快了伤口愈合过程。该结构可在未来的临床研究中与其他促进伤口愈合的药物联合使用，作为一种安全的结构。
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