School of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia.
Department of Chemistry, The University of Adelaide, Adelaide, SA, 5005, Australia.
Chem Soc Rev. 2023 Nov 27;52(23):8295-8318. doi: 10.1039/d3cs00509g.
Imaging techniques permit the study of the molecular interactions that underlie health and disease. Each imaging technique collects unique chemical information about the cellular environment. Multimodal imaging, using a single probe that can be detected by multiple imaging modalities, can maximise the information extracted from a single cellular sample by combining the results of different imaging techniques. Of particular interest in biological imaging is the combination of the specificity and sensitivity of optical fluorescence microscopy (OFM) with the quantitative and element-specific nature of X-ray fluorescence microscopy (XFM). Together, these techniques give a greater understanding of how native elements or therapeutics affect the cellular environment. This review focuses on recent studies where both techniques were used in conjunction to study cellular systems, demonstrating the breadth of biological models to which this combination of techniques can be applied and the potential for these techniques to unlock untapped knowledge of disease states.
成像技术可以研究构成健康和疾病基础的分子相互作用。每种成像技术都收集有关细胞环境的独特化学信息。使用可以被多种成像模式检测到的单个探针的多模态成像，可以通过结合不同成像技术的结果，从单个细胞样本中提取最大信息。在生物成像中特别感兴趣的是将光学荧光显微镜（OFM）的特异性和灵敏度与 X 射线荧光显微镜（XFM）的定量和元素特异性性质相结合。这些技术一起可以更深入地了解天然元素或治疗剂如何影响细胞环境。这篇综述重点介绍了最近使用这两种技术结合研究细胞系统的研究，展示了可以应用这种技术组合的生物模型的广泛范围，以及这些技术有可能揭示疾病状态的未开发知识。
