微生物群分析揭示了PHF11在激素调节的子宫内膜异位囊肿发展中的潜在机制。

Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of -regulated endometriotic cysts.

作者信息

Hu Jingjing, Li Guanjian, Huang Miaomiao, Shen Qunshan, Gu Hao, Xue Nairui, Zhang Junqiang, Xu Xiaofeng, Yang Wenjuan, Xing Aying, Wu Xiao, Wang Ying, Wu Rong, Xu Chuan, Li Yuqian, Fang Yuan, Cao Yunxia

机构信息

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No 218 Jixi Road, Hefei, Anhui 230022, China.

NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei, Anhui 230032, China.

出版信息

iScience. 2023 Oct 7;26(11):108158. doi: 10.1016/j.isci.2023.108158. eCollection 2023 Nov 17.

DOI:10.1016/j.isci.2023.108158

PMID:37915596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10616313/

Abstract

Endometriosis (EMS) is a prevalent disease and the etiologies has not uniform. Microbiota is associated with human diseases. To delve into the relationship between EMS and microbiota, Ectopic (EM) and eutopic (EU) endometrial tissues, pharyngeal swabs, and stools were collected from EMS patients. The microbiota composition of EM and EU partially overlapped, with similar taxon numbers and diversity, but the richness levels were significantly different. A comparison of intestinal microbes in healthy individuals (FN) and EMS patients (FE) revealed that the richness of Enterococcus, Pseudomonas, Haemophilus, and Neisseria was enhanced in FE. In addition, Enterococcus-induced mice (EFA) presented with a higher degree of lesion infiltration and a wider distribution of lesions. Proteomic analysis revealed the expression of plant homeodomain finger 11 (PHF11) was notably downregulated in EFA. And the downregulated expression of PHF11 was accompanied by the upregulated expression of interleukin 8 (IL-8). Our findings suggest a potential regulatory mechanism for PHF11 in EMS development.

摘要

子宫内膜异位症（EMS）是一种常见疾病，其病因并不统一。微生物群与人类疾病有关。为了深入研究EMS与微生物群之间的关系，收集了EMS患者的异位（EM）和在位（EU）子宫内膜组织、咽拭子和粪便。EM和EU的微生物群组成部分重叠，分类单元数量和多样性相似，但丰富度水平存在显著差异。对健康个体（FN）和EMS患者（FE）的肠道微生物进行比较发现，FE中肠球菌、假单胞菌、嗜血杆菌和奈瑟菌的丰富度增加。此外，肠球菌诱导的小鼠（EFA）表现出更高程度的病变浸润和更广泛的病变分布。蛋白质组学分析显示，EFA中植物同源结构域手指蛋白11（PHF11）的表达明显下调。PHF11表达下调伴随着白细胞介素8（IL-8）表达上调。我们的研究结果提示了PHF11在EMS发生发展中的潜在调控机制。