Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China.
School of Marine Sciences, China University of Geosciences, Beijing 100083, PR China.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006142.
A novel mesophilic, chemolithoautotrophic, hydrogen-oxidizing bacterium, designated strain ST1-3, was isolated from mud sediment samples collected from mangroves in Jiulong River estuary. The cells were Gram-stain-negative, non-motile and rod-shaped. The temperature, pH and salinity ranges for growth of strain ST1-3 were 4-45 °C (optimum, 35 °C), pH 5.0-8.5 (optimum, pH 7.0) and 0-8.0 % (w/v) NaCl (optimum, 4.0 %). The isolate was an obligate chemolithoautotroph capable of growth using hydrogen as the only energy source, and molecular oxygen, thiosulphate and elemental sulphur as electron acceptors. The major cellular fatty acids of strain ST1-3 were summed feature 3 (C 7 and/or C 6), C and summed feature 8 (C 7). The major polar lipids were phosphatidylethanolamine, phosphatidyldimethyl ethanolamine and phosphatidylglycerol. The respiratory quinone was menaquinone-6. The genomic DNA G+C content was 43.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and core genes showed that the novel isolate belonged to the genus and was most closely related to 42BKT (94.7 % sequence identity). The average nucleotide identity and digital DNA-DNA hybridization values between ST1-3 and 42BKT were 74.6 and 16.3 %, respectively. On the basis of the phenotypic, phylogenetic and genomic data presented here, strain ST1-3 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain ST1-3 (=MCCC M25234=KCTC 25639).
一株新型的嗜中温、化能自养、氢氧化细菌，被命名为 ST1-3 菌株，从九龙江河口红树林的泥沉积物样本中分离得到。细胞革兰氏阴性，不运动，呈杆状。ST1-3 菌株的生长温度、pH 值和盐度范围分别为 4-45°C（最佳温度为 35°C）、pH5.0-8.5（最佳 pH 值为 7.0）和 0-8.0%（w/v）NaCl（最佳为 4.0%）。该分离株是一种严格的化能自养型细菌，只能以氢气作为唯一能源，并以分子氧、硫代硫酸盐和元素硫作为电子受体进行生长。ST1-3 菌株的主要细胞脂肪酸是总和特征 3（C7 和/或 C6）、C 和总和特征 8（C7）。主要极性脂类是磷脂酰乙醇胺、磷脂酰二甲乙醇胺和磷脂酰甘油。呼吸醌是menaquinone-6。基因组 DNA G+C 含量为 43.6 mol%。基于 16S rRNA 基因序列和核心基因的系统发育分析表明，新型分离株属于 属，与 42BKT（94.7%序列同一性）最为密切相关。ST1-3 与 42BKT 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 74.6%和 16.3%。根据这里提出的表型、系统发育和基因组数据，ST1-3 株代表了属的一个新型物种，建议采用新种名 sp. nov.，其模式菌株为 ST1-3（=MCCC M25234=KCTC 25639）。
