Jiang Fan, Hao Xun, Li Ding, Zhu Xuying, Huang Jiamei, Lai Qiliang, Wang Jianning, Wang Liping, Shao Zongze
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China, Xiamen 361102, China.
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361102, China.
Microorganisms. 2024 Aug 4;12(8):1584. doi: 10.3390/microorganisms12081584.
A novel Gram-stain-negative, facultatively anaerobic, and mixotrophic bacterium, designated as strain LZ166, was isolated from the bathypelagic seawater in the western Pacific Ocean. The cells were short rod-shaped, oxidase- and catalase-positive, and motile by means of lateral flagella. The growth of strain LZ166 was observed at 10-45 °C (optimum 34-37 °C), at pH 5-10 (optimum 6-8), and in the presence of 0-5% NaCl (optimum 1-3%). A phylogenetic analysis based on the 16S rRNA gene showed that strain LZ166 shared the highest similarity (98.58%) with B7 and formed a distinct branch within the genus. The genomic characterization, including average nucleotide identity (ANI, 90.73-76.79%), average amino identity (AAI, 88.50-79.03%), and digital DNA-DNA hybridization (dDDH, 36.1-22.2%) values between LZ166 and other species within the genus, further substantiated its novelty. The genome of strain LZ166 was 6,119,659 bp in size with a 64.7 mol% DNA G+C content. The predominant fatty acid was summed feature 8 (C7 and/or C6). The major polar lipids identified were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), glycolipid (GL), and phosphatidylglycerol (PG), with ubiquinone-10 (Q-10) as the predominant respiratory quinone. The genomic annotation indicated the presence of genes for a diverse metabolic profile, including pathways for carbon fixation via the Calvin-Benson-Bassham cycle and inorganic sulfur oxidation. Based on the polyphasic taxonomic results, strain LZ166 represented a novel species of the genus , for which the name sp. nov. is proposed, with the type strain LZ166 (=MCCC M28807 = KACC 23148 = KCTC 82889).
从西太平洋深海海水分离出一株新型革兰氏阴性、兼性厌氧和混合营养细菌，命名为LZ166菌株。细胞呈短杆状，氧化酶和过氧化氢酶呈阳性，借助侧生鞭毛运动。LZ166菌株在10-45℃（最适34-37℃）、pH 5-10（最适6-8）和0-5%NaCl（最适1-3%）存在的条件下生长。基于16S rRNA基因的系统发育分析表明，LZ166菌株与B7的相似性最高（98.58%），并在该属内形成一个独特的分支。基因组特征，包括LZ166与该属内其他物种之间的平均核苷酸同一性（ANI，90.73-76.79%）、平均氨基酸同一性（AAI，88.50-79.03%）和数字DNA-DNA杂交（dDDH，36.1-22.2%）值进一步证实了其新颖性。LZ I66菌株的基因组大小为6,119,659 bp，DNA G+C含量为64.7 mol%。主要脂肪酸为总和特征8（C₁₇:₇和/或C₁₆:₆）。鉴定出的主要极性脂为双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、糖脂（GL）和磷脂酰甘油（PG），以泛醌-10（Q-10）作为主要呼吸醌。基因组注释表明存在多种代谢途径的基因．包括通过卡尔文-本森-巴斯姆循环进行碳固定和无机硫氧化的途径。基于多相分类学结果，LZ166菌株代表该属的一个新物种，为此提出新物种名称sp. nov.，模式菌株为LZ166（=MCCC M28807 = KACC 23148 = KCTC 82889）。
