Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
School of Chemical New Materials Engineering, Shandong Polytechnic College, Jining 272027, China.
Anal Methods. 2020 Jun 26;12(24):3123-3129. doi: 10.1039/d0ay00404a.
As one of the chemical weapons in World War I, phosgene (COCl2) is an extremely toxic gas. Due to wide applications in industrial production, phosgene can easily leak inadvertently and poses a serious threat to the environment and human health and safety. In this study, we report for the first time a new fluorescent probe (bis-(1H-benzimidazol-2-yl)-methanone) for the rapid detection of phosgene. The probe is based on the easily prepared bis-(1H-benzimidazol-2-yl), which can quickly combine with phosgene to obtain a six-membered cyclic carbamylation product showing fluorescence turn-on. The combination of phosgene caused the maximum absorption of the probe shifting from 361 nm to 373 nm, which showed a color change from colorless to yellow under visible light. Meanwhile, a strong fluorescence emission peak appeared at 428 nm, which showed change from no fluorescence to blue-violet fluorescence under a UV lamp (365 nm). The response of the probe towards phosgene is fast (within 30 s), highly selective and sensitive (the detection limit in solution being 3.3 nM). Furthermore, the portable test strips manufactured using the probe can conveniently perform visual and fluorescence detection of phosgene in the gas phase.
光气（COCl2）作为一战时期的化学武器之一，是一种毒性极强的气体。由于在工业生产中广泛应用，光气很容易被意外泄漏，对环境和人类健康及安全构成严重威胁。在本研究中，我们首次报道了一种用于快速检测光气的新型荧光探针（双（1H-苯并咪唑-2-基）甲酮）。该探针基于易于制备的双（1H-苯并咪唑-2-基），可与光气快速结合，得到呈现荧光开启的六元环状氨基甲酰化产物。光气的结合导致探针的最大吸收从 361nm 移动到 373nm，在可见光下显示出从无色到黄色的颜色变化。同时，在 428nm 处出现强荧光发射峰，在 365nm 的紫外灯下显示出从无荧光到蓝紫色荧光的变化。该探针对光气的响应快速（在 30 秒内）、选择性高且灵敏度高（在溶液中的检测限为 3.3 nM）。此外，使用该探针制造的便携式试条可方便地对气相中的光气进行目视和荧光检测。
