Liu Bin, Wu Huafeng, Cao Yinzhu, Ma Guanpeng, Zheng Xiaowen, Zhu Haoxiang, Song Xingrong, Sui Shunzhao
Chongqing Engineering Research Center for Floriculture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Institute of Horticulture, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.
Front Plant Sci. 2024 Nov 20;15:1491246. doi: 10.3389/fpls.2024.1491246. eCollection 2024.
Flower characteristics are crucial ornamental and reproductive traits in . Over its long cultivation history, variations have been observed in the floral organs, primarily in the petals, with limited reports on stamen traits. Stamen variation, integral to the mating system, can enhance the plant's ornamental value and directly impact its reproductive success.
This study is the first to report the phenomenon of red coloration in stamens. Using UPLC-MS/MS, we analyzed the types and quantities of major metabolites in stamens of different colors.
Our results indicated that the red coloration was primarily due to the accumulation 42 on of high levels of anthocyanins, specifically cyanidin 3-O-rutinoside and cyanidin 3-O-glucoside. Transcriptomic sequencing identified 63 differentially expressed genes (DEGs) related to the anthocyanin biosynthetic pathway, most showing peak expression during the bud stage. The results of the metabolite analysis and transcriptomic sequencing were similar to those of previous studies on petal reddening, suggesting a close relationship between the mechanisms of stamen and petal reddening.
This study elucidated the mechanism of stamen reddening in , expanding the species' genetic resources and offering insights into color changes across floral tissue..
花朵特征是[植物名称]重要的观赏和繁殖性状。在其漫长的栽培历史中，人们观察到花器官存在变异，主要发生在花瓣上，而关于雄蕊性状的报道较少。雄蕊变异是交配系统的一部分，可提高植物的观赏价值并直接影响其繁殖成功率。
本研究首次报道了[植物名称]雄蕊变红的现象。我们使用超高效液相色谱-串联质谱法（UPLC-MS/MS）分析了不同颜色雄蕊中主要代谢物的类型和含量。
我们的结果表明，红色主要是由于高水平花青素的积累，特别是矢车菊素3-O-芸香糖苷和矢车菊素3-O-葡萄糖苷。转录组测序鉴定出63个与花青素生物合成途径相关的差异表达基因（DEG），大多数在芽期表达达到峰值。代谢物分析和转录组测序的结果与先前关于花瓣变红的研究结果相似，表明雄蕊和花瓣变红机制之间存在密切关系。
本研究阐明了[植物名称]雄蕊变红的机制，扩展了该物种的遗传资源，并为整个花组织的颜色变化提供了见解。
