Al-Zahrani Maryam H, Alghamdi Rana A, Tarbiah Nesrin I, Alkhattabi Nuha A, Joharjy Husam M, Khalifa Reham A
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Department of Chemistry, Sciences and Arts College, King Abdulaziz University, Rabigh, Saudi Arabia.
Saudi J Biol Sci. 2023 Nov;30(11):103821. doi: 10.1016/j.sjbs.2023.103821. Epub 2023 Sep 27.
SARS-CoV-2, which initiated the worldwide COVID-19 epidemic in 2019, has rapidly emerged and spread, resulting in significant public health challenges worldwide. The COVID-19 severity signs and their association with specific genes have been investigated to better comprehend this phenomenon. In this study, several genes were investigated to see whether they correspond with COVID-19 sickness severity. This research aims to determine and evaluate certain gene expression levels associated with the immune system, as these genes were reported to play important roles in immune control during the COVID-19 outbreak. We analyzed two immunity-linked genes: and in COVID-19 patients' samples using RT-PCR, compared them to the samples from recovered, immunized, and healthy individuals. These data were examined to determine the potential relationships between clinical patterns, illness severity, and progression, and SARS-CoV-2 infection immunology. We observed that gene expression was higher in COVID-19 vaccinated and control groups, but lower in active and recovered COVID-19 patients. On the other hand, gene expression was elevated in infected and recovered COVID-19 groups. According to our study, the proteins CD27 and SAMHD1 are essential for controlling the immunological response to COVID-19. Changes in their expression levels could increase the susceptibility of patients to severe complications associated with the disease. Therefore, the gene expression level of these proteins could serve as viable prognostic markers for COVID-19.
2019年引发全球新冠疫情的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）迅速出现并传播，给全球公共卫生带来了重大挑战。为了更好地理解这一现象，人们对新冠病毒疾病的严重程度体征及其与特定基因的关联进行了研究。在本研究中，对多个基因进行了调查，以确定它们是否与新冠病毒疾病的严重程度相关。这项研究旨在确定和评估与免疫系统相关的某些基因表达水平，因为据报道这些基因在新冠疫情期间的免疫控制中发挥着重要作用。我们使用逆转录聚合酶链反应（RT-PCR）分析了新冠患者样本中的两个与免疫相关的基因，并将其与康复者、接种疫苗者和健康个体的样本进行比较。对这些数据进行了分析，以确定临床模式、疾病严重程度和病程与SARS-CoV-2感染免疫学之间的潜在关系。我们观察到，在接种新冠疫苗的人群和对照组中，某基因的表达较高，但在新冠病毒活跃感染者和康复者中较低。另一方面，在新冠病毒感染组和康复组中，另一基因的表达有所升高。根据我们的研究，CD27和SAMHD1蛋白对于控制新冠病毒的免疫反应至关重要。它们表达水平的变化可能会增加患者发生与该疾病相关的严重并发症的易感性。因此，这些蛋白的基因表达水平可作为新冠病毒疾病可行的预后标志物。
