Central Research Laboratory, Siberian State Medical University, Tomsk, Russia.
Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
Biomed Res Int. 2020 Jul 1;2020:1242364. doi: 10.1155/2020/1242364. eCollection 2020.
A functional interplay between BAs and microbial composition in gut is a well-documented phenomenon. In bile, this phenomenon is far less studied, and with this report, we describe the interactions between the BAs and microbiota in this complex biological matrix. . Thirty-seven gallstone disease patients of which twenty-one with infection were enrolled in the study. The bile samples were obtained during laparoscopic cholecystectomy for gallstone disease operative treatment. Common bile acid composition was measured by LC-MS/MS. Gallbladder microbiota were previously analyzed with 16S rRNA gene sequencing on Illumina MiSeq platform. The associations between bile acid composition and microbiota were analyzed.
Bile acid signature and infection status exert influence on beta-diversity of bile microbial community. Direct correlations were found between taurocholic acid, taurochenodeoxycholic acid concentrations, and alpha-diversity of bile microbiota. Taurocholic acid and taurochenodeoxycholic acid both show positive associations with the presence of Chitinophagaceae family, Microbacterium and Lutibacterium genera, and Prevotella intermedia. Also, direct associations were identified for taurocholic acid concentration and the presence of Actinomycetales and Bacteroidales orders, Lautropia genus, Jeotgalicoccus psychrophilus, and Haemophilus parainfluenzae as well as for taurochenodeoxycholic acid and Acetobacteraceae family and Sphingomonas genus. There were no differences in bile acid concentrations between infected and noninfected patients. . Associations between diversity, taxonomic profile of bile microbiota, and bile acid levels were evidenced in patients with cholelithiasis. Increase of taurochenodeoxycholic acid and taurocholic acid concentration correlates with bile microbiota alpha-diversity and appearance of opportunistic pathogens.
胆汁酸（BAs）与肠道微生物组成之间的功能相互作用是一个已有充分文献记录的现象。在胆汁中，这一现象的研究要少得多，通过本报告，我们描述了这种复杂生物基质中胆汁酸与微生物群之间的相互作用。 . 本研究纳入了 37 名胆囊疾病患者，其中 21 名患有 感染。在腹腔镜胆囊切除术治疗胆囊疾病手术过程中获得胆汁样本。采用 LC-MS/MS 测定胆汁中胆汁酸的组成。先前使用 Illumina MiSeq 平台对 16S rRNA 基因测序分析了胆囊微生物群。分析了胆汁酸组成与微生物群之间的关联。
. 结果：胆汁酸特征和 感染状态对胆汁微生物群落的β多样性有影响。发现牛磺胆酸、牛磺鹅脱氧胆酸浓度与胆汁微生物群落的α多样性之间存在直接相关性。牛磺胆酸和牛磺鹅脱氧胆酸均与胆石病患者中奇古菌科、微杆菌属和路特氏菌属和中间普雷沃氏菌的存在呈正相关。此外，还发现牛磺胆酸浓度与放线菌目和拟杆菌目、洛特氏菌属、嗜冷杰氏球菌和副流感嗜血杆菌的存在以及牛磺鹅脱氧胆酸与醋杆菌科和鞘氨醇单胞菌属之间存在直接关联。感染和未感染患者的胆汁酸浓度无差异。. 在胆石病患者中证实了胆汁微生物群的多样性、分类群谱与胆汁酸水平之间存在关联。牛磺鹅脱氧胆酸和牛磺胆酸浓度的增加与胆汁微生物群的α多样性和机会性病原体的出现相关。
