Department of Breast Diseases, Guangdong Women and Children Hospital, Guangzhou, China.
Translational Medicine Center, Guangdong Women and Children Hospital, Guangzhou, China.
J Int Med Res. 2024 Jan;52(1):3000605231220827. doi: 10.1177/03000605231220827.
We aimed to explore the prognostic value of DNA methylation in breast cancer.
Breast cancer patients with and without recurrence or metastasis and matched non-breast cancer patients were screened retrospectively from 2014 to 2016. Bisulfite conversion and fluorescence quantitative methylation-specific polymerase chain reaction were used to detect the methylation status and distribution levels in patient breast tissues.
DNA methylation was more frequent in breast cancer tissues than in non-breast cancer tissues, but was not significantly correlated with any relevant breast cancer patient clinicopathological characteristic. methylation rates were higher in patients with recurrence or metastasis. methylation, tumor size, lymph node status, and progesterone receptor (PR) expression could influence prognosis. methylation was significantly associated with worse disease-free survival in breast cancer patients, with receiver operating characteristic curve analysis indicating that it had good prognostic ability, with an area under the curve (AUC) value of 0.719. The AUC values increased when methylation was combined with tumor size, lymph node status, and PR to predict prognosis.
DNA methylation was an independent predictors of breast cancer prognostic risk. This could possibly help improve comprehensive prognosis prediction methods when combined with other risk factors.
探讨 DNA 甲基化在乳腺癌中的预后价值。
本研究回顾性筛选了 2014 年至 2016 年间无复发或转移和有复发或转移的乳腺癌患者及匹配的非乳腺癌患者。采用亚硫酸氢盐转化和荧光定量甲基化特异性聚合酶链反应检测患者乳腺组织中的甲基化状态和分布水平。
与非乳腺癌组织相比，乳腺癌组织中 DNA 甲基化更为常见，但与任何相关的乳腺癌患者临床病理特征均无显著相关性。复发或转移患者的甲基化率更高。甲基化、肿瘤大小、淋巴结状态和孕激素受体（PR）表达可影响预后。DNA 甲基化与乳腺癌患者无病生存显著相关，受试者工作特征曲线分析表明其具有良好的预后能力，曲线下面积（AUC）值为 0.719。当将甲基化与肿瘤大小、淋巴结状态和 PR 结合起来预测预后时，AUC 值增加。
DNA 甲基化是乳腺癌预后风险的独立预测因子。与其他危险因素结合，可能有助于改善综合预后预测方法。
