OF4949，氨肽酶B的新型抑制剂。I. 分类学、发酵、分离与特性鉴定

OF4949, new inhibitors of aminopeptidase B. I. Taxonomy, fermentation, isolation and characterization.

作者信息

Sano S, Ikai K, Kuroda H, Nakamura T, Obayashi A, Ezure Y, Enomoto H

出版信息

J Antibiot (Tokyo). 1986 Dec;39(12):1674-84. doi: 10.7164/antibiotics.39.1674.

DOI:10.7164/antibiotics.39.1674

Abstract

New aminopeptidase B inhibitors that we named OF4949-I, II, III and IV were isolated from the culture broth of a fungus, Penicillium rugulosum OF4949. The molecular formula of I was C23H26N4O8 and that of II, C22H24O8, judging from elemental analysis and secondary ion mass spectrometry. The concentrations of I, II, III and IV required for 50% inhibition of aminopeptidase, using Ehrlich ascites carcinoma cells as the source of the enzyme, were 0.0054, 0.0048, 3.4 and 1.7 micrograms/ml, respectively. Components I and II augmented delayed-type hypersensitivity in mice to sheep red blood cells.

摘要

我们从一种真菌——皱褶青霉OF4949的培养液中分离出了新的氨肽酶B抑制剂，命名为OF4949-I、II、III和IV。根据元素分析和二次离子质谱分析，I的分子式为C23H26N4O8，II的分子式为C22H24O8。以艾氏腹水癌细胞作为酶的来源，I、II、III和IV对氨肽酶50%抑制所需的浓度分别为0.0054、0.0048、3.4和1.7微克/毫升。成分I和II增强了小鼠对绵羊红细胞的迟发型超敏反应。