Siddi Giuliana, Piras Francesca, Meloni Maria Pina, Gymoese Pernille, Torpdahl Mia, Fredriksson-Ahomaa Maria, Migoni Mattia, Cabras Daniela, Cuccu Mario, De Santis Enrico Pietro Luigi, Scarano Christian
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
Department of Bacteria, Parasites & Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark.
Foods. 2023 Dec 23;13(1):65. doi: 10.3390/foods13010065.
The objective of this investigation was to evaluate and prevalence in wild boars hunted in Sardinia and further characterize the isolates and analyse antimicrobial resistance (AMR) patterns. In order to assess slaughtering hygiene, an evaluation of carcasses microbial contamination was also carried out. Between 2020 and 2022, samples were collected from 66 wild boars hunted during two hunting seasons from the area of two provinces in northern and central Sardinia (Italy). Samples collected included colon content samples, mesenteric lymph nodes samples and carcass surface samples. and detection was conducted on each sample; also, on carcass surface samples, total aerobic mesophilic count and count were evaluated. On and isolates, antimicrobial susceptibility was tested and whole genome sequencing was applied. was identified in the colon content samples of 3/66 (4.5%) wild boars; isolates were subs. , ser. and subs. . was detected from 20/66 (30.3%) wild boars: in 18/66 (27.3%) colon contents, in 3/66 (4.5%) mesenteric lymph nodes and in 3/49 (6.1%) carcass surface samples. In all, 24 isolates were analysed and 20 different sequence types were detected, with the most common being ST860. Regarding AMR, no resistance was detected in isolates, while expected resistance towards -lactams ( gene) and streptogramin ( gene) was observed in isolates (91.7% and 4.2%, respectively). The low presence of AMR is probably due to the low anthropic impact in the wild areas. Regarding the surface contamination of carcasses, values (mean ± standard deviation log CFU/cm) were 2.46 ± 0.97 for ACC and 1.07 ± 1.18 for . The results of our study confirm that wild boars can serve as reservoirs and spreaders of and ; the finding of presence on carcass surface highlights how meat may become superficially contaminated, especially considering that contamination is linked to the conditions related to the hunting, handling and processing of game animals.
本调查的目的是评估撒丁岛猎杀的野猪中[病原体名称]的感染情况和流行率，并进一步鉴定分离株并分析其抗菌药物耐药性（AMR）模式。为了评估屠宰卫生状况，还对胴体的微生物污染进行了评估。在2020年至2022年期间，从意大利撒丁岛北部和中部两个省份地区的两个狩猎季节猎杀的66头野猪中采集了样本。采集的样本包括结肠内容物样本、肠系膜淋巴结样本和胴体表面样本。对每个样本进行了[病原体名称]检测；此外，对胴体表面样本，评估了总需氧嗜温菌数和[另一种微生物名称]数。对[病原体名称]和[另一种微生物名称]分离株进行了抗菌药物敏感性测试并应用了全基因组测序。在3/66（4.5%）的野猪结肠内容物样本中鉴定出了[病原体名称]；分离株为[病原体名称亚种名称]亚种，[血清型名称]血清型和[病原体名称亚种名称]亚种。从20/66（30.3%）的野猪中检测到了[另一种微生物名称]：在18/66（27.3%）的结肠内容物中、3/（66）（4.5%）的肠系膜淋巴结中以及3/49（6.1%）的胴体表面样本中。总共分析了24株[另一种微生物名称]分离株，检测到20种不同的序列类型，最常见的是ST860。关于抗菌药物耐药性，在[病原体名称]分离株中未检测到耐药性，而在[另一种微生物名称]分离株中观察到对β-内酰胺类（[基因名称]基因）和链阳菌素（[基因名称]基因）的预期耐药性（分别为91.7%和4.2%）。抗菌药物耐药性的低发生率可能是由于野生区域的人类影响较小。关于胴体的表面污染，总需氧嗜温菌数（平均±标准差log CFU/cm）的值为2.46±0.97，[另一种微生物名称]的值为1.07±1.18。我们的研究结果证实，野猪可以作为[病原体名称]和[另一种微生物名称]的储存宿主和传播者；在胴体表面发现[另一种微生物名称]表明肉可能会受到表面污染，特别是考虑到污染与野生动物的狩猎、处理和加工条件有关。
